FceR1 alpha Recombinant Antibody | FceRIa recombinant antibody

Anti-FceR1 alpha [Mar-1]

Cat. Num. AAA488485

• Gene Names: Fcer1a; FcERI; Fce1a; Fcr-5; fcepsilonri
• Reactivity: Mouse
• Applications: Immunoprecipitation, Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Protein A Affinity Purified
• Synonyms: FceR1 alpha; Monoclonal Recombinant Antibody; Anti-FceR1 alpha [Mar-1]; Fcer1a; Fc epsilon RI alpha; FceRI alpha; FceRI-alpha; high affinity IgE receptor; High affinity immunoglobulin epsilon receptor subunit alpha;Fce1a; Fc-epsilon RI-alpha; FcERI; IgE Fc receptor subunit alpha; FceR1; FceRIa recombinant antibody

• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Hamster (Armenian) IgG lambda
• Clone Number: Mar-1
• Specificity: This antibody specifically targets the non-signalling alpha chain of the FceR1 complex.
• Purity/Purification: Protein A Affinity Purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 250

• Applicable Applications for FceRIa recombinant antibody: Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Deplete
• Antibody Type: Recombinant Antibody
• Antibody Species: Armenian Hamster
• Original Species Name: Armenian Hamster
• Clone Note: This antibody has been used in flow cytometric analysis of FceR1 alpha cell surface expression by basophils (Min et al, 2004; Chen et al, 2009), and immunohistochemical analysis of epidermal sheets (Holzmann et al, 2004). It has also been used in immunoprecipitation analysis of cell lysates from lung cDCs and MC/9 mast cells (Grayson et al, 2007). In vitro incubation of basophils with this antibody blocks subsequent MAR-1 staining, but does not activate basophil IL-4 production (Sokol et al, 2008). Intravenous treatment of mice with this antibody results in basophil depletion in peripheral blood, spleen, bone marrow and liver, but not skin or intraperitoneal mast cell depletion (Sokol et al, 2008). Following treatment with this antibody, basophil-depleted mice display impaired Th2 differentiation upon papain immunisation (Sokol et al, 2008). However, MAR-1 treated mice respond normally to high-dose intravenous Ig in both the K/BxN serum transfer arthritis and collagen-induced arthritis models, despite basophil depletion (Campbell et al, 2014).
• Buffer Composition: PBS only with 0.02% Proclin 300.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Flow Cytometry (FC/FACS)

(Flow-cytometry using the anti-FceR1 alpha antibody Mar-1 Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of Mar-1 at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)

Related Product Information for FceRIa recombinant antibody

Recombinant monoclonal antibody to FceR1 alpha. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the hybridoma Mar-1.

Product Categories/Family for FceRIa recombinant antibody

Allergy; Basophils

References

Holzmann et al. A model system using tape stripping for characterization of Langerhans cell-precursors in vivo. J Invest Dermatol. 2004 May;122(5):1165-74. PMID:15140219
Original Published Journal: J Invest Dermatol. 2004 May;122(5):1165-74.
Publication Note: Describes the original use of this antibody in immunohistochemical analysis of epidermal sheets.

NCBI and Uniprot Product Information

• NCBI GI #: 226823365
• NCBI GeneID: 14125
• NCBI Accession #: NP_034314.2
• NCBI GenBank Nucleotide #: NP_034314.2
• UniProt Accession #: P20489
• NCBI Official Full Name: high affinity immunoglobulin epsilon receptor subunit alpha
• NCBI Official Synonym Full Names: Fc receptor, IgE, high affinity I, alpha polypeptide
• NCBI Official Symbol: Fcer1a
• NCBI Official Synonym Symbols: FcERI; Fce1a; Fcr-5; fcepsilonri
• NCBI Protein Information: high affinity immunoglobulin epsilon receptor subunit alpha
• UniProt Protein Name: High affinity immunoglobulin epsilon receptor subunit alpha
• UniProt Gene Name: Fcer1a
• UniProt Synonym Gene Names: Fce1a; FcERI

Uniprot Description

Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.