Rabbit anti-Human Probable ATP-dependent RNA helicase DDX5 Polyclonal Antibody | anti-DDX5 antibody

Rabbit anti-human Probable ATP-dependent RNA helicase DDX5 polyclonal Antibody(DDX5)

Cat. Num. AAA1494949

• Gene Names: DDX5; p68; HLR1; G17P1; HUMP68
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: Probable ATP-dependent RNA helicase DDX5; Polyclonal Antibody; Rabbit anti-human Probable ATP-dependent RNA helicase DDX5 polyclonal Antibody(DDX5); DEAD box protein 5; RNA helicase p68; DDX5; G17P1; HELR; HLR1; anti-DDX5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 614

• Applicable Applications for anti-DDX5 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: WB:1:500-2000; IHC:1:20-1:200
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human Probable ATP-dependent RNA helicase DDX5 protein (1-290aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-26045 / sc-26043 / sc-32858
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(All lanes: Probable ATP-dependent RNA helicase DDX5 antibody at 3ug/mlLane 1:NIH3T3 whole cell lysateLane 2:Hela whole cell lysateLane 3:293T whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 70,61 kDaObserved band size: 69 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human breast cancer using MBS1494949 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human tonsil using MBS1494949 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney using MBS1494949 at dilution 1:100)

Related Product Information for anti-DDX5 antibody

Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for estrogen receptor ESR1 and androgen receptor AR. Increases ESR1 AF-1 domain-mediated transactivation and ESR1 AF-1 and AF-2 domains transcriptional synergistic activity. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specicic manner; the function probbaly involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms.

NCBI and Uniprot Product Information

• NCBI GI #: 4758138
• NCBI GeneID: 1655
• NCBI Accession #: NP_004387.1
• NCBI GenBank Nucleotide #: NM_004396.3
• UniProt Accession #: P17844; O75681; Q53Y61; B4DLW8; B5BU21; D3DU32; E7ETL9
• Molecular Weight: 60,563 Da
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX5
• NCBI Official Synonym Full Names: DEAD-box helicase 5
• NCBI Official Symbol: DDX5
• NCBI Official Synonym Symbols: p68; HLR1; G17P1; HUMP68
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX5
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX5
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX5
• UniProt Synonym Gene Names: G17P1; HELR; HLR1
• UniProt Entry Name: DDX5_HUMAN

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

DDX5: a putative ATP-dependent RNA helicase. A proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Belongs to the DEAD box helicase family. DDX5/DDX17 subfamily. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly.

Protein type: Nucleolus; EC 3.6.4.13; RNA-binding; RNA splicing; Spliceosome; Nuclear receptor co-regulator; Helicase

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: membrane; nucleolus; nucleoplasm; nucleus; ribonucleoprotein complex

Molecular Function: androgen receptor binding; ATP binding; ATP-dependent RNA helicase activity; calcium-dependent protein binding; calmodulin binding; enzyme binding; estrogen receptor binding; protein binding; RNA helicase activity; transcription coactivator activity

Biological Process: cell growth; circadian rhythm; in utero embryonic development; mRNA transcription; negative regulation of transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; positive regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of estrogen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; regulation of alternative nuclear mRNA splicing, via spliceosome; regulation of osteoblast differentiation; regulation of viral genome replication; RNA secondary structure unwinding