Mouse anti-Human Collagen Type IV Monoclonal Antibody

Collagen Type IV

Cat. Num. AAA344048

• Reactivity: Human
• Synonyms: Collagen Type IV; Monoclonal Antibody; anti-Collagen Type IV antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Form/Format: Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide

• Application Notes: The recommended working dilution range is 1:100 - 1:200.
• Positive Control: Lung, Muscle
• Principles and Procedures: Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.
• Reconstitution: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.; The concentrated antibody requires dilution in the optimized buffer, to the recommended working dilution range.
• Specimen Collection and Preparation for Analysis: Each tissue section should be fixed with 10% neutral buffered formalin, cut to the applicable thickness (4um), and placed on a glass slide that is positively charged. The prepared slide may then be baked for a minimum of 30 minutes in a 53-65 degree C oven (do not exceed 24 hours).; Note: Performance evaluation has been shown on human tissues only. Variable results may occur due to extended fixation time or special processes of specific tissue preparations.
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.; To ensure proper stability and delivery of the antibody after each run, replace the cap and immediately place the bottle in a refrigerator in an upright position.; Positive and negative controls should be simultaneously run with unknown specimens.

Immunohistochemistry (IHC)

(Collagen Type IV [IHC549] on Liver)

Related Product Information for anti-Collagen Type IV antibody

Intended Use: The Collagen Type IV [IHC549] antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test methods.
Description: Collagen Type IV is a primary component in the basal lamina that is used as a marker to observe the presence of the lamina and examine its structure. In addition to the epithelial basal lamina, Anti-Collagen Type IV stains mesenchymal components. It is useful for identifying soft tissue cancers including schwannomas and leiomyomas. Anti-Collagen Type IV frequently reacts with these tissues after becoming well-differentiated and malignant. The use of Anti-Collagen Type IV produces more reliable results than non-specific silver reticulum stains when investigating the vascular elements of neoplasms, hemangiopericytoma, angiosarcoma and epithelioid hemangioendothelioma.

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