Human Collagen Type XI Alpha 1 ELISA Kit | COL11alpha1 elisa kit

Human Collagen Type XI Alpha 1 ELISA Kit

Cat. Num. AAA763194

• Gene Names: COL11A1; STL2; COLL6; CO11A1
• Reactivity: Human
• Synonyms: Collagen Type XI Alpha 1; Human Collagen Type XI Alpha 1 ELISA Kit; COL11alpha1/COL11A1/CO11A1/COLL6/STL2; COL11alpha1 elisa kit

• Reactivity: Human
• Sequence Length: 1767

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 39.063-2500pg/ml
• Sensitivity: 23.438pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for COL11alpha1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human COL11alpha1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human COL11alpha1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human COL11alpha1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human COL11alpha1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 6165881
• NCBI GeneID: 1301
• Molecular Weight: 167,752 Da
• NCBI Official Full Name: collagen type XI alpha-1
• NCBI Official Synonym Full Names: collagen type XI alpha 1
• NCBI Official Symbol: COL11A1
• NCBI Official Synonym Symbols: STL2; COLL6; CO11A1
• NCBI Protein Information: collagen alpha-1(XI) chain
• UniProt Protein Name: Collagen alpha-1(XI) chain
• UniProt Gene Name: COL11A1
• UniProt Synonym Gene Names: COLL6
• UniProt Entry Name: COBA1_HUMAN

NCBI Description

This gene encodes one of the two alpha chains of type XI collagen, a minor fibrillar collagen. Type XI collagen is a heterotrimer but the third alpha chain is a post-translationally modified alpha 1 type II chain. Mutations in this gene are associated with type II Stickler syndrome and with Marshall syndrome. A single-nucleotide polymorphism in this gene is also associated with susceptibility to lumbar disc herniation. Multiple transcript variants have been identified for this gene. [provided by RefSeq, Nov 2009]

Uniprot Description

COL11A1: May play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils. Defects in COL11A1 are the cause of Stickler syndrome type 2 (STL2); also known as Stickler syndrome vitreous type 2. STL2 is an autosomal dominant form of Stickler syndrome, an inherited disorder that associates ocular signs with more or less complete forms of Pierre Robin sequence, bone disorders and sensorineural deafness. Ocular disorders may include juvenile cataract, myopia, strabismus, vitreoretinal or chorioretinal degeneration, retinal detachment, and chronic uveitis. Robin sequence includes an opening in the roof of the mouth (a cleft palate), a large tongue (macroglossia), and a small lower jaw (micrognathia). Bones are affected by slight platyspondylisis and large, often defective epiphyses. Juvenile joint laxity is followed by early signs of arthrosis. The degree of hearing loss varies among affected individuals and may become more severe over time. Syndrome expressivity is variable. Defects in COL11A1 are the cause of Marshall syndrome (MRSHS). It is an autosomal dominant disorder characterized by ocular abnormalities, deafness, craniofacial anomalies, and anhidrotic ectodermal dysplasia. Clinical features include short stature; flat or retruded midface with short, depressed nose, flat nasal bridge and anteverted nares; cleft palate with or without the Pierre Robin sequence; appearance of large eyes with ocular hypertelorism; cataracts, either congenital or juvenile; esotropia; high myopia; sensorineural hearing loss; spondyloepiphyseal abnormalities; calcification of the falx cerebri; ectodermal abnormalities, including defects in sweating and dental structures. Defects in COL11A1 are the cause of fibrochondrogenesis type 1 (FBCG1). A severe short-limbed skeletal dysplasia characterized by broad long-bone metaphyses, pear-shaped vertebral bodies, and characteristic morphology of the growth plate, in which the chondrocytes have a fibroblastic appearance and there are regions of fibrous cartilage extracellular matrix. Clinical features include a flat midface with a small nose and anteverted nares, significant shortening of all limb segments but relatively normal hands and feet, and a small bell-shaped thorax with a protuberant abdomen. Belongs to the fibrillar collagen family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: collagen type XI; endoplasmic reticulum lumen; extracellular region

Molecular Function: extracellular matrix binding; extracellular matrix structural constituent; metal ion binding; protein binding, bridging

Biological Process: cartilage condensation; chondrocyte development; collagen catabolic process; collagen fibril organization; detection of mechanical stimulus involved in sensory perception of sound; embryonic skeletal morphogenesis; extracellular matrix disassembly; extracellular matrix organization and biogenesis; inner ear morphogenesis; ossification; proteoglycan metabolic process; sensory perception of sound; ventricular cardiac muscle morphogenesis; visual perception

Disease: Fibrochondrogenesis 1; Intervertebral Disc Disease; Marshall Syndrome; Stickler Syndrome, Type Ii