Mouse MPO ELISA Kit | MPO elisa kit

Mouse MPO PicoKine ELISA Kit

Cat. Num. AAA176536

• Gene Names: Mpo; mKIAA4033
• Reactivity: Mouse
• Synonyms: MPO; Mouse MPO PicoKine ELISA Kit; Myeloperoxidase; myeloperoxidase; 84 kDa myeloperoxidase; 89 kDa myeloperoxidase; MPO Myeloperoxidase; Myeloperoxidase heavy chain; Myeloperoxidase light chain; PERM_HUMAN; MPO elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Mpo
• Sequence Length: 718

• Samples: Cell culture supernates, cell lysates, serum and plasma(heparin, edta)
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess interassay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MPO elisa kit

Principle of the Assay: The Picokine™ Mouse Mpo Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Mpo with a 96-well strip plate that is pre-coated with antibody specific for Mpo. The detection antibody is a biotinylated antibody specific for Mpo. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Mpo with immunogen: Expression system for standard: NSO; Immunogen sequence: M16-T718. The kit is analytically validated with ready to use reagents. To measure Mouse Mpo, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Mpo in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Mpo in the sample.

Background/Introduction: Myeloperoxidase(MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function.

NCBI and Uniprot Product Information

• NCBI GI #: 341941245
• NCBI GeneID: 17523
• UniProt Accession #: P11247; Q5NCP1
• Molecular Weight: 81,182 Da
• NCBI Official Full Name: Myeloperoxidase
• NCBI Official Synonym Full Names: myeloperoxidase
• NCBI Official Symbol: Mpo
• NCBI Official Synonym Symbols: mKIAA4033
• NCBI Protein Information: myeloperoxidase
• UniProt Protein Name: Myeloperoxidase
• Protein Family: Myeloperoxidase
• UniProt Gene Name: Mpo
• UniProt Synonym Gene Names: MPO
• UniProt Entry Name: PERM_MOUSE

Uniprot Description

MPO: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain. Belongs to the peroxidase family. XPO subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.11.2.2; Oxidoreductase

Cellular Component: extracellular space; azurophil granule; mitochondrion; lysosome; nucleus; secretory granule

Molecular Function: heparin binding; peroxidase activity; metal ion binding; heme binding; oxidoreductase activity

Biological Process: respiratory burst during defense response; defense response to Gram-positive bacterium; removal of superoxide radicals; hydrogen peroxide catabolic process; response to yeast; defense response; response to oxidative stress; defense response to fungus