Mouse anti-Human FADS1 Monoclonal Antibody | anti-FADS1 antibody

Mouse Monoclonal [clone 2D9] (IgG2a,k) to Human FADS1

Cat. Num. AAA246045

• Gene Names: FADS1; D5D; TU12; FADS6; FADSD5; LLCDL1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: FADS1; Monoclonal Antibody; Mouse Monoclonal [clone 2D9] (IgG2a; k) to Human FADS1; Anti-FADS1 Antibody (clone 2D9) IHC-plus; D5D; Delta-5 fatty acid desaturase; Delta(5) fatty acid desaturase; Delta-5 desaturase; Fatty acid desaturase 1; FADSD5; LLCDL1; Delta(5) desaturase; TU12; Human FADS1; anti-FADS1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2D9
• Specificity: Human FADS1
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 501

• Applicable Applications for anti-FADS1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000); Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using cell line lysates and the recombinant protein used as the immunogen.
• Target Species: Human
• Immunogen Description: (NP_037534, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: FADS1 antibody was raised against (NP_037534, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-FADS1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(monoclonal antibody, clone 2D9. Western blot of expression in HeLa NE.)

ELISA (EIA)

(Detection limit for recombinant GST tagged FADS1 is approximately 0.03 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 214831730
• NCBI GeneID: 3992
• NCBI Accession #: NP_037534.3
• NCBI GenBank Nucleotide #: NM_013402.4
• UniProt Accession #: O60427; Q53GM5; Q8N3A6; Q8NCC7; Q8NCG0; Q96I39; Q96SV3; Q96T10; Q9NRP8; Q9NYX1; A8K0I7; B2RAI0
• Molecular Weight: 42,474 Da
• NCBI Official Full Name: fatty acid desaturase 1
• NCBI Official Synonym Full Names: fatty acid desaturase 1
• NCBI Official Symbol: FADS1
• NCBI Official Synonym Symbols: D5D; TU12; FADS6; FADSD5; LLCDL1
• NCBI Protein Information: fatty acid desaturase 1; delta-5 desaturase; delta(5) desaturase; delta-5 fatty acid desaturase; delta(5) fatty acid desaturase; linoleoyl-CoA desaturase (delta-6-desaturase)-like 1
• UniProt Protein Name: Fatty acid desaturase 1
• Protein Family: Fatty acid desaturase
• UniProt Gene Name: FADS1
• UniProt Synonym Gene Names: FADSD5; D5D; Delta(5) desaturase; Delta-5 desaturase
• UniProt Entry Name: FADS1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. [provided by RefSeq, Jul 2008]

Uniprot Description

FADS1: Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the desaturation of dihomo-gamma-linoleic acid (DHGLA) (20:3n-6) and eicosatetraenoic acid (20:4n-3) to generate arachidonic acid (AA) (20:4n-6) and eicosapentaenoic acid (EPA)(20:5n-3) respectively. Belongs to the fatty acid desaturase family.

Protein type: EC 1.14.19.-; Membrane protein, multi-pass; Oxidoreductase; Lipid Metabolism - unsaturated fatty acid biosynthesis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q12.2-q13.1

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; mitochondrion; membrane; integral to membrane

Molecular Function: C-5 sterol desaturase activity; iron ion binding; heme binding; oxidoreductase activity

Biological Process: icosanoid biosynthetic process; regulation of transcription, DNA-dependent; cell-cell signaling; regulation of cell differentiation; unsaturated fatty acid metabolic process; linoleic acid metabolic process; unsaturated fatty acid biosynthetic process; cellular lipid metabolic process; cellular response to starvation; phospholipid biosynthetic process