Bone Morphogenetic Protein 8B Recombinant Protein | BMP8B recombinant protein

Recombinant Bone Morphogenetic Protein 8B (BMP8B)

Cat. Num. AAA2030305

• Gene Names: BMP8B; OP2; BMP8
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Bone Morphogenetic Protein 8B; Recombinant Bone Morphogenetic Protein 8B (BMP8B); BMP8B recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; G GHLSHPPHVF PQRRLGVREP RDMQREIREVLGLPGRPRSR APVGAAQQPA SAPLFMLDLY RAMTDDSGGG TPQPHLDRADLIMSFVNIVE RDRTLGYQEP HWKEFHFDLT QIPAGEAVTA AEFRIYKEPSTHPLNTTLHI SMFEVVQEHS NRESDLFFLD LQTLRSGDEG WLVLDITAASDRWLLNHHKD LGLRLYVETE DGHSIDPGLA GLLGRQAPRS RQPFMVGFFRANQSPVRAPR TARPLKKKQL NQINQLPHSN KHLGILDDGH GSHGREVCRRHELYVSFRDL GWLDSVIAPQ GYSAYYCAGE CIYPLNSCMN STNHATMQALVHLMKPDIIP KVCCVPTELS AISLLYYDRN NNVILRRERN MVVQACGCH
• Sequence Length: 402

• Applicable Applications for BMP8B recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 46.7kDa
• Accurate Molecular Mass (KD): 47kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Gly20~His399
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 32250692
• NCBI GeneID: 656
• Molecular Weight: 39,151 Da
• NCBI Official Full Name: bone morphogenetic protein 8B
• NCBI Official Synonym Full Names: bone morphogenetic protein 8b
• NCBI Official Symbol: BMP8B
• NCBI Official Synonym Symbols: OP2; BMP8
• NCBI Protein Information: bone morphogenetic protein 8B
• UniProt Protein Name: Bone morphogenetic protein 8B
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP8B
• UniProt Synonym Gene Names: BMP8; BMP-8; BMP-8B; OP-2
• UniProt Entry Name: BMP8B_HUMAN

NCBI Description

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has led to speculation of possible bone inductive activity. [provided by RefSeq, Jul 2008]

Uniprot Description

BMP8B: Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. Belongs to the TGF-beta family.

Protein type: Cytokine; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p35-p32

Cellular Component: extracellular space

Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: regulation of apoptosis; BMP signaling pathway; ossification; cartilage development; regulation of MAPKKK cascade; cell development; skeletal development; growth