Rat IGFBP5 ELISA Kit | IGFBP5 elisa kit

Rat IGFBP5 PicoKine ELISA Kit

Cat. Num. AAA177250

• Gene Names: Igfbp5; IGF-BP5
• Reactivity: Rat
• Synonyms: IGFBP5; Rat IGFBP5 PicoKine ELISA Kit; Insulin-like growth factor-binding protein 5; insulin-like growth factor binding protein 5; IBP 5; IBP-5; IBP5; IBP5_HUMAN; IGF binding protein 5; IGF BP5; IGF-binding protein 5; IGFBP 5; IGFBP-5; Insulin like growth factor binding protein 5; IGFBP5 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant Rat Igfbp5
• Sequence Length: 271

• Samples: Cell culture supernates, cell lysates, serum and plasma (heparin, edta)
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess interassay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IGFBP5 elisa kit

Principle of the Assay: The Picokine™ Rat Igfbp5 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Igfbp5 with a 96-well strip plate that is pre-coated with antibody specific for Igfbp5. The detection antibody is a biotinylated antibody specific for Igfbp5. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Rat Igfbp5 with immunogen: Expression system for standard: NSO; Immunogen sequence: L20-E271. The kit is analytically validated with ready to use reagents. To measure Rat Igfbp5, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Rat Igfbp5 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Igfbp5 in the sample.

Background/Introduction: Insulin-like growth factor-binding protein 5 is a protein that in humans is encoded by the IGFBP5 gene. The IGFBP5 gene was mapped to chromosome 2q33-q34. The expression of IGFBP5 by stable transfection and adenovirus-mediated infection was inhibitory to growth in 2 human breast cancer cell lines. Stable expression of IGFBP5 in the breast cancer cell lines also inhibited the formation and growth of tumors following injection in athymic mice. IGFBP5 is believed a growth inhibitor and proapoptotic agent in breast cancer cells.

NCBI and Uniprot Product Information

• NCBI GI #: 124070
• NCBI GeneID: 25285
• UniProt Accession #: P24594
• Molecular Weight: 30,298 Da
• NCBI Official Full Name: Insulin-like growth factor-binding protein 5
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 5
• NCBI Official Symbol: Igfbp5
• NCBI Official Synonym Symbols: IGF-BP5
• NCBI Protein Information: insulin-like growth factor-binding protein 5; IBP-5; IGFBP-5; IGF-binding protein 5
• UniProt Protein Name: Insulin-like growth factor-binding protein 5
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: Igfbp5
• UniProt Synonym Gene Names: Igfbp-5; IBP-5; IGF-binding protein 5; IGFBP-5
• UniProt Entry Name: IBP5_RAT

NCBI Description

may be involved in ovarian folliculogenesis and may play a modulatory role in type I fiber-dominated muscles [RGD, Feb 2006]

Uniprot Description

IGFBP5: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Protein type: Secreted; Secreted, signal peptide; Cell cycle regulation

Cellular Component: extracellular space

Molecular Function: insulin-like growth factor binding; insulin-like growth factor I binding; fibronectin binding; insulin-like growth factor II binding

Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; mammary gland involution; hair follicle morphogenesis; regulation of insulin-like growth factor receptor signaling pathway; negative regulation of smooth muscle cell proliferation; negative regulation of insulin-like growth factor receptor signaling pathway; glucose metabolic process; negative regulation of smooth muscle cell migration; glucose homeostasis; osteoblast differentiation; positive regulation of protein kinase B signaling cascade; regulation of growth; negative regulation of translation; striated muscle cell differentiation; negative regulation of osteoblast differentiation; skeletal muscle growth; regulation of cell growth; negative regulation of cell migration