Typical Testing Data/Standard Curve (for reference only)
Kanamycin ELISA Kit
Kanamycin Competitive ELISA Kit
Synonyms
Kanamycin; Kanamycin Competitive ELISA Kit; Kanamycin elisa kit
Preparation and Storage
Upon receipt, store Anti-Kanamycin Antibody (500X), Secondary Antibody HRP Conjugate (1000X), Kanamycin Standard, Kanamycin Conjugate, and 100X Conjugate Diluent at -20 degree C. Store all the remaining components at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Kanamycin elisa kit
Principle of the Assay: First, a kanamycin conjugate is coated on an ELISA plate. The unknown kanamycin samples or kanamycin standards are then added to the kanamycin conjugate preabsorbed ELISA plate. After a brief incubation, an anti-kanamycin monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The total content of kanamycin in unknown extracted samples is determined by comparison with a kanamycin standard curve.
Background/Introduction: Kanamycin (also known as Kanamycin A) is an antibiotic used in the treatment of dangerous bacterial infections such as tuberculosis. It is typically administered by mouth, injection into a vein, or injection into a muscle. Kanamycin is recommended for short-term dosing from 7 to 10 days and is ineffective in viral infections. Common side effects include problems with hearing and balance as well as kidney problems. Kanamycin belongs to the aminoglycoside family of medications and it blocks the production of proteins that are required for bacterial survival. Specifically, it binds to the bacterial ribosomal 30S subunit which results in improper alignment with mRNA and causes the wrong amino acid to be synthesized into the peptide. As a result, nonfunctional peptide chains are produced leading to bacterial death. Kanamycin is commonly used as a selective agent by molecular biologists to grow bacteria (e.g. E. coli) which have taken up expression plasmids (circular DNA) also containing a gene that codes for kanamycin resistance (usually Neomycin phosphotransferase II [NPT II/Neo]). Bacteria that have taken up and express the exogenous kanamycin resistance gene are grown on kanamycin containing luria broth (LB) agar plates or are grown in liquid LB media containing kanamycin. Only the bacteria expressing the kanamycin resistance gene can resist the selection conditions and grow. The Kanamycin Competitive ELISA Kit provides a convenient method for the detection of total kanamycin in extracts from cells, tissue, serum, plasma, or foods. The total content of kanamycin in unknown samples is determined by comparison with a kanamycin standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples. The kit has a detection sensitivity limit of 7.8 nM kanamycin.
Background/Introduction: Kanamycin (also known as Kanamycin A) is an antibiotic used in the treatment of dangerous bacterial infections such as tuberculosis. It is typically administered by mouth, injection into a vein, or injection into a muscle. Kanamycin is recommended for short-term dosing from 7 to 10 days and is ineffective in viral infections. Common side effects include problems with hearing and balance as well as kidney problems. Kanamycin belongs to the aminoglycoside family of medications and it blocks the production of proteins that are required for bacterial survival. Specifically, it binds to the bacterial ribosomal 30S subunit which results in improper alignment with mRNA and causes the wrong amino acid to be synthesized into the peptide. As a result, nonfunctional peptide chains are produced leading to bacterial death. Kanamycin is commonly used as a selective agent by molecular biologists to grow bacteria (e.g. E. coli) which have taken up expression plasmids (circular DNA) also containing a gene that codes for kanamycin resistance (usually Neomycin phosphotransferase II [NPT II/Neo]). Bacteria that have taken up and express the exogenous kanamycin resistance gene are grown on kanamycin containing luria broth (LB) agar plates or are grown in liquid LB media containing kanamycin. Only the bacteria expressing the kanamycin resistance gene can resist the selection conditions and grow. The Kanamycin Competitive ELISA Kit provides a convenient method for the detection of total kanamycin in extracts from cells, tissue, serum, plasma, or foods. The total content of kanamycin in unknown samples is determined by comparison with a kanamycin standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples. The kit has a detection sensitivity limit of 7.8 nM kanamycin.
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Product Notes
The Kanamycin (Catalog #AAA169597) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Kanamycin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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