Typical Testing Data/Standard Curve (for reference only)
1-Methyladenosine (m1A) ELISA Kit | m1A elisa kit
1-Methyladenosine (m1A) ELISA Kit
Synonyms
1-Methyladenosine (m1A); 1-Methyladenosine (m1A) ELISA Kit; m1A elisa kit
Assay Type
Quantitative Competitive
Preparation and Storage
Upon receipt, aliquot and store the Anti-1-Methyladenosine Antibody and the m1A Standard at -20 degree C and the m1A Conjugate at -80 degree C to avoid multiple freeze/thaw cycles. Store all other components at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for m1A elisa kit
Principle of the Assay: The 1-Methyladenosine (m1A) ELISA kit is a competitive ELISA for the quantitative measurement 1-methyladenosine (m1A). The unknown 1-methyladenosine samples or 1-methyladenosine standards are first added to a 1-methyladenosine-BSA conjugate preabsorbed microplate. After a brief incubation, an anti-1-methyladenosine monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The 1-methyladenosine content in unknown samples is determined by comparison with predetermined 1-methyladenosine standard curve.
Background/Introduction: Modifications to ribonucleic acids (RNAs) are integrally involved in the function, integrity, metabolism, and biosynthesis of RNA molecules. There are over 100 RNA modifications that have been discovered with the majority occurring in transfer RNA (tRNA), but they can also be found in ribosomal RNA (rRNA), small nuclear RNA (snRNA), messenger (mRNA), and long non-coding RNA (ncRNA). Urinary excretion of modified nucleosides has been linked to a variety of cancers, AIDS, and other malignant disease states. 1-methyladenosine (m1A) is a prominent reversible post-transcriptional RNA modification that influences the function and structure of tRNA and rRNA. 1-Methyladenosine occurs at positions 9, 14, and 58 of tRNA, with position 58 (m1A58) being important to tRNA stabilization. Methyltransferases catalyze the addition of a methyl group to the first position nitrogen on the adenosine base structure, resulting in the addition of a positive charge to the molecule. The reaction is reversible with demethylases such as ALKBH3. 1-methyladenosine can affect ribosomal biogenesis, antibiotic resistance in bacteria, react to environmental stress in tRNA, and interfere with Watson-Crick base pairing, which ultimately affects reverse transcription and protein translation. Urine samples of cancer, rheumatoid arthritis, and AIDS patients have all demonstrated high levels of m1A, which supports its role as a functional detection biomarker. Elevated serum levels of 1-methyladenosine have been detected under stress conditions. Recent 1-methyladenosine research is uncovering new roles and mechanisms involving 1-methyladenosine. While m1dA in DNA is considered a form of damage leading to genomic mutations, and thus requiring repair, endogenous enzymes proliferate the m1A modification in RNA. The m1A at position 9 (m1A9) is known to stabilize the canonical cloverleaf motif in tRNA. In addition, the m1A58 of tRNA is present in all eukaryotic types which may imply it is vital to molecular structure and stability. While 1-methyladenosine has been identified as an early malignant disease marker, its complete role as a modified nucleoside has yet to be elucidated. The 1-Methyladenosine (m1A) ELISA Kit is a competitive enzyme immunoassay developed for rapid detection and quantitation of 1-methyladenosine in urine, serum, or plasma samples. The quantity of 1-methyladenosine in unknown samples is determined by comparing its absorbance with that of a known 1-methyladenosine standard curve. The kit has a 1-methyladenosine detection sensitivity of approximately 2 ng/mL. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.
Background/Introduction: Modifications to ribonucleic acids (RNAs) are integrally involved in the function, integrity, metabolism, and biosynthesis of RNA molecules. There are over 100 RNA modifications that have been discovered with the majority occurring in transfer RNA (tRNA), but they can also be found in ribosomal RNA (rRNA), small nuclear RNA (snRNA), messenger (mRNA), and long non-coding RNA (ncRNA). Urinary excretion of modified nucleosides has been linked to a variety of cancers, AIDS, and other malignant disease states. 1-methyladenosine (m1A) is a prominent reversible post-transcriptional RNA modification that influences the function and structure of tRNA and rRNA. 1-Methyladenosine occurs at positions 9, 14, and 58 of tRNA, with position 58 (m1A58) being important to tRNA stabilization. Methyltransferases catalyze the addition of a methyl group to the first position nitrogen on the adenosine base structure, resulting in the addition of a positive charge to the molecule. The reaction is reversible with demethylases such as ALKBH3. 1-methyladenosine can affect ribosomal biogenesis, antibiotic resistance in bacteria, react to environmental stress in tRNA, and interfere with Watson-Crick base pairing, which ultimately affects reverse transcription and protein translation. Urine samples of cancer, rheumatoid arthritis, and AIDS patients have all demonstrated high levels of m1A, which supports its role as a functional detection biomarker. Elevated serum levels of 1-methyladenosine have been detected under stress conditions. Recent 1-methyladenosine research is uncovering new roles and mechanisms involving 1-methyladenosine. While m1dA in DNA is considered a form of damage leading to genomic mutations, and thus requiring repair, endogenous enzymes proliferate the m1A modification in RNA. The m1A at position 9 (m1A9) is known to stabilize the canonical cloverleaf motif in tRNA. In addition, the m1A58 of tRNA is present in all eukaryotic types which may imply it is vital to molecular structure and stability. While 1-methyladenosine has been identified as an early malignant disease marker, its complete role as a modified nucleoside has yet to be elucidated. The 1-Methyladenosine (m1A) ELISA Kit is a competitive enzyme immunoassay developed for rapid detection and quantitation of 1-methyladenosine in urine, serum, or plasma samples. The quantity of 1-methyladenosine in unknown samples is determined by comparing its absorbance with that of a known 1-methyladenosine standard curve. The kit has a 1-methyladenosine detection sensitivity of approximately 2 ng/mL. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.
Product Categories/Family for m1A elisa kit
Similar Products
Product Notes
The m1A (Catalog #AAA169566) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "1-Methyladenosine (m1A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
