Typical Testing Data/Standard Curve (for reference only)
Lipid Assay Kit
Lipid Quantitation Kit (Colorimetric)
Synonyms
Lipid; Lipid Quantitation Kit (Colorimetric); Lipid assay kit
Preparation and Storage
-20 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Lipid assay kit
Background/Introduction: Lipids are a diverse group of molecules that include monoglycerides, diglycerides, trigylcerides, fats, sterols, and others. Not only do lipids define and preserve cellular membrane integrity, but they are also involved in cellular processes such as membrane trafficking, signal transduction, apoptosis, and energy storage. Perturbation in the metabolism of lipids has been linked to many diseases such as cancer, diabetes, Alzheimer's disease, and coronary heart disease. In order to study lipids, they must often be extracted first from tissues or cellular cultures (1) and then quantified. Some methods for lipid quantification have been described that are quite sensitive, however they require expensive equipment such as an HPLC machine, light scattering detection technology, or a latroscan TLC-FID analyser (2,3). Lipid Quantification Kit measures the lipid content (unsaturated fatty acids only) of samples using the sulfo-phospho-vanillin method (4), resulting in a simple colorimetric readout amenable to multi-well plate detection. First, a crude or purified lipid source is applied to a 96 well plate. Then concentrated sulfuric acid is added and the samples are heated to solubilize and prime the total lipid sample, followed by the addition of vanillin in an acid solution. The lipids react with vanillin in the presence of the acids to form a colorimetric product that is easily detected on a microplate reader. Each kit provides sufficient reagents to perform 100 assays including standards and unknown samples.
Product Categories/Family for Lipid assay kit
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Product Notes
The Lipid (Catalog #AAA168217) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Lipid, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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