14-3-3 gamma recombinant protein

14-3-3 gamma, 1-247aa, Human, E Coli

Cat. Num. AAA203050

• Gene Names: YWHAG; EIEE56; PPP1R170; 14-3-3GAMMA
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: 14-3-3 gamma; 1-247aa; Human; E Coli; Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein gamma polypeptide; YWHAG; Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; gamma polypeptide; Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein; gamma polypeptide 14 3 3 protein gamma; 14 3 3 protein gamma subtype; 14 3 3gamma; 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide; KCIP 1; KCIP1; Protein kinase C inhibitor protein 1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide.; 14-3-3 gamma recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid in phosphate buffered saline (pH 7.4), 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MVDREQLVQK ARLAEQAERY DDMAAAMKNV TELNEPLSNE ERNLLSVAYK NVVGARRSSW RVISSIEQKT SADGNEKKIE MVRAYREKIE KELEAVCQDV LSLLDNYLIK NCSETQYESK VFYLKMKGDY YRYLAEVATG EKRATVVESS EKAYSEAHEI SKEHMQPTHP IRLGLALNYS VFYYEIQNAP EQACHLAKTA FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDD DGGEGNN
• Sequence Length: 247

• Applicable Applications for 14-3-3 gamma recombinant protein: SDS-PAGE
• Antigen Species: Human
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for 14-3-3 gamma recombinant protein

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon,sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3gamma, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. Recombinant human YWHAG was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for 14-3-3 gamma recombinant protein

Signal Transduction

References

Chen XQ, et al. (2005) Glia. 42 (4):315-24 ; Chen XQ, Yu AC, et al. (2002) Biochem Biophys Res Commun. 296 (3):657-63 ;

NCBI and Uniprot Product Information

• NCBI GI #: 21464101
• NCBI GeneID: 7532
• NCBI Accession #: NP_036611
• NCBI GenBank Nucleotide #: NM_012479.3
• UniProt Accession #: P61981; O70457; P35214; Q6FH52; Q9UDP2; Q9UN99
• Molecular Weight: 28 kDa (247 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: 14-3-3 protein gamma
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma
• NCBI Official Symbol: YWHAG
• NCBI Official Synonym Symbols: EIEE56; PPP1R170; 14-3-3GAMMA
• NCBI Protein Information: 14-3-3 protein gamma
• UniProt Protein Name: 14-3-3 protein gamma
• UniProt Gene Name: YWHAG
• UniProt Synonym Gene Names: KCIP-1

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]

Uniprot Description

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.