Tissue factor ELISA Kit | F3 elisa kit

Mouse Tissue factor/F3 PicoKine ELISA Kit

Cat. Num. AAA177533

• Gene Names: F3; TF; Cf3; Cf-3; CD142; AA409063
• Reactivity: Mouse. There is no detectable cross-reactivity with other relevant proteins.
• Synonyms: Tissue factor; Mouse Tissue factor/F3 PicoKine ELISA Kit; CD142; CD142 antigen; Coagulation factor III (thromboplastin tissue factor); Coagulation factor III; F3; FLJ17960; TF; TF_HUMAN; TFA; Thromboplastin; coagulation factor III (thromboplastin; tissue factor); F3 elisa kit

• Reactivity: Mouse. There is no detectable cross-reactivity with other relevant proteins.
• Sequence Length: 294

• Samples: Cell culture supernates, serum, plasma (heparin, EDTA, citrate) and Cell lysates
• Assay Type: Sandwich
• Sensitivity: <10pg/ml
• Immunogen: NSO, A29-E251
• Intra-Assay Precision: Sample 1: n=16; Mean(pg/ml): 113; Standard deviation: 4.41; CV(%): 3.9 Sample 2: n=16; Mean(pg/ml): 327; Standard deviation: 14.4; CV(%): 4.4 Sample 3: n=16; Mean(pg/ml): 603; Standard deviation: 30.8; CV(%): 5.1
• Inter-Assay Precision: Sample 1: n=24; Mean(pg/ml): 120; Standard deviation: 5.64; CV(%): 4.7 Sample 2: n=24; Mean(pg/ml): 315; Standard deviation: 18.3; CV(%): 5.8 Sample 3: n=24; Mean(pg/ml): 592; Standard deviation: 37.3; CV(%): 6.3
• Assay Range: 15.6pg/ml-1000pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse Tissue Factor/F3 PicoKine ELISA Kit standard curve )

Related Product Information for F3 elisa kit

Description: Sandwich ELISA kit of Quantitative Detection for Mouse Tissue factor/F3
Background: Tissue factor, also called platelet tissue factor, factor III, thrombokinase, or CD142 is a protein present in sub endothelial tissue and leukocytes necessary for the initiation of thrombin formation from the zymogene prothrombin. Tissue factor gene was mapped to 1pter-p21 by study of somatic cell hybrids with a species-specific sensitive chromogenic assay. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII.

NCBI and Uniprot Product Information

• NCBI GI #: 170172540
• NCBI GeneID: 14066
• NCBI Accession #: NP_034301.3
• NCBI GenBank Nucleotide #: NM_010171.3
• UniProt Accession #: P20352
• Molecular Weight: 32,935 Da
• NCBI Official Full Name: tissue factor
• NCBI Official Synonym Full Names: coagulation factor III
• NCBI Official Symbol: F3
• NCBI Official Synonym Symbols: TF; Cf3; Cf-3; CD142; AA409063
• NCBI Protein Information: tissue factor
• UniProt Protein Name: Tissue factor
• Protein Family: Tissue factor
• UniProt Gene Name: F3
• UniProt Synonym Gene Names: Cf-3; Cf3; TF
• UniProt Entry Name: TF_MOUSE

NCBI Description

This gene encodes a membrane-bound glycoprotein that forms the primary physiological initiator of the blood coagulation process following vascular damage. The encoded protein binds to coagulation factor VIIa and the ensuing complex catalyzes the proteolytic activation of coagulation factors IX and X. Mice lacking encoded protein die in utero resulting from massive hemorrhaging in both extraembryonic and embryonic vessels. A severe deficiency of the encoded protein in mice results in impaired uterine homeostasis, shorter life spans due to spontaneous fatal hemorrhages and cardiac fibrosis. [provided by RefSeq, Aug 2015]

Uniprot Description

F3: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII. TF expression is highly dependent upon cell type. TF can also be induced by the inflammatory mediators interleukin 1 and TNF-alpha, as well as by endotoxin, to appear on monocytes and vascular endothelial cells as a component of cellular immune response. Lung, placenta and pancreas. Belongs to the tissue factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface

Cellular Component: extracellular matrix; extracellular space; cell surface; membrane; cytoplasm; integral to membrane; plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protease binding; phospholipid binding

Biological Process: caspase activation; positive regulation of protein kinase B signaling cascade; positive regulation of angiogenesis; activation of plasma proteins during acute inflammatory response; hemostasis; cytokine and chemokine mediated signaling pathway; positive regulation of endothelial cell proliferation; blood coagulation; positive regulation of smooth muscle cell migration