Mouse anti-Human Insulin Monoclonal Antibody | anti-INS antibody

Insulin (ILPR, Insulin, IRDN) (MaxLight 490)

Cat. Num. AAA6245679

• Gene Names: INS; ILPR; IRDN; IDDM2; MODY10
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified by immunoaffinity chromatography.
• Synonyms: Insulin; Monoclonal Antibody; Insulin (ILPR; IRDN) (MaxLight 490); anti-INS antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 10F646
• Specificity: Recognizes human Insulin.
• Purity/Purification: Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-INS antibody: Immunohistochemistry (IHC)
• Immunogen: Biosynthetic human insulin.
• Conjugate: MaxLight490
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Immunohistochemistry (IHC)

(Immunohistochemistry Analysis: Fig. 1 Paraffin-embedded human pancreas tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1.25 ug/mL concentration of I7660-09C. Reactivity was detected using the IHC-Select Detection kit. Staining pattern appears as expected. Fig.2 Negative control staining of human pancreas tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Negative control reagent was used in place of the antibody. Same detection method was used as in figure one. No signal detected.)

Product Categories/Family for anti-INS antibody

Antibodies; Insulin

NCBI and Uniprot Product Information

• NCBI GI #: 386828
• NCBI GeneID: 3630
• UniProt Accession #: P01308; Q5EEX2
• Molecular Weight: 11,981 Da
• NCBI Official Full Name: insulin
• NCBI Official Synonym Full Names: insulin
• NCBI Official Symbol: INS
• NCBI Official Synonym Symbols: ILPR; IRDN; IDDM2; MODY10
• NCBI Protein Information: insulin; proinsulin; preproinsulin
• UniProt Protein Name: Insulin
• Protein Family: Insulin
• UniProt Gene Name: INS
• UniProt Entry Name: INS_HUMAN

NCBI Description

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Uniprot Description

Insulin: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Heterodimer of a B chain and an A chain linked by two disulfide bonds. Belongs to the insulin family.

Protein type: Secreted, signal peptide; Hormone; Secreted

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular space; Golgi lumen; endoplasmic reticulum lumen; extracellular region

Molecular Function: insulin-like growth factor receptor binding; identical protein binding; protein binding; protease binding; hormone activity; insulin receptor binding

Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of vasodilation; glucose homeostasis; activation of NF-kappaB transcription factor; positive regulation of glucose import; negative regulation of protein oligomerization; regulation of protein localization; cell-cell signaling; negative regulation of gluconeogenesis; acute-phase response; regulation of transmembrane transporter activity; negative regulation of NAD(P)H oxidase activity; positive regulation of mitosis; positive regulation of nitric-oxide synthase activity; activation of protein kinase B; negative regulation of acute inflammatory response; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of protein amino acid autophosphorylation; positive regulation of cell growth; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of protein catabolic process; regulation of amino acid metabolic process; positive regulation of cell differentiation; negative regulation of glycogen catabolic process; wound healing; positive regulation of cellular protein metabolic process; positive regulation of glycogen biosynthetic process; glucose transport; regulation of protein secretion; regulation of transcription, DNA-dependent; negative regulation of lipid catabolic process; negative regulation of vasodilation; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; negative regulation of proteolysis; negative regulation of protein secretion; MAPKKK cascade; negative regulation of fatty acid metabolic process; alpha-beta T cell activation; endocrine pancreas development; positive regulation of protein kinase B signaling cascade; G-protein coupled receptor protein signaling pathway; cellular protein metabolic process; fatty acid homeostasis; positive regulation of glycolysis; insulin receptor signaling pathway; energy reserve metabolic process; positive regulation of insulin receptor signaling pathway; regulation of insulin secretion; positive regulation of DNA replication; positive regulation of cytokine secretion; positive regulation of cell migration

Disease: Diabetes Mellitus, Insulin-dependent, 2; Diabetes Mellitus, Permanent Neonatal; Hyperproinsulinemia; Maturity-onset Diabetes Of The Young, Type 10