Human 14-3-3 protein eta (YWHAH) ELISA Kit | YWHAH elisa kit

Human 14-3-3 protein eta (YWHAH)

Cat. Num. AAA766089

• Gene Names: YWHAH; YWHA1
• Reactivity: Human
• Synonyms: 14-3-3 protein eta (YWHAH); Human 14-3-3 protein eta (YWHAH); YWHA1/Protein AS1; YWHAH elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of 14-3-3 protein eta. No significant cross-reactivity or interference between 14-3-3 protein eta and analogues was observed.
• Sequence Length: 246

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.625-40ng/ml
• Sensitivity: 0.375ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 14- 3-3 protein eta were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 14- 3-3 protein eta were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: 4 degree C for 6 months

Typical Standard Curve

Related Product Information for YWHAH elisa kit

Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 14-3-3 protein eta. During the reaction, 14-3-3 protein eta in the sample or standard competes with a fixed amount of 14-3-3 protein eta on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to 14-3-3 protein eta. Excess conjugate and unbound sample or standard are washed from the plate, and HRPStreptavidin( SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 14-3-3 protein eta in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4507951
• NCBI GeneID: 7533
• NCBI Accession #: NP_003396.1
• NCBI GenBank Nucleotide #: NM_003405.3
• Molecular Weight: 28,219 Da
• NCBI Official Full Name: 14-3-3 protein eta
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta
• NCBI Official Symbol: YWHAH
• NCBI Official Synonym Symbols: YWHA1
• NCBI Protein Information: 14-3-3 protein eta
• UniProt Protein Name: 14-3-3 protein eta
• Protein Family: 14-3-3 protein
• UniProt Gene Name: YWHAH
• UniProt Synonym Gene Names: YWHA1
• UniProt Entry Name: 1433F_HUMAN

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder. [provided by RefSeq, Jun 2009]

Uniprot Description

14-3-3 eta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes.

Protein type: Adaptor/scaffold; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 22q12.3

Cellular Component: cytoplasm; cytoplasmic vesicle membrane; cytosol; mitochondrion; plasma membrane

Molecular Function: actin binding; enzyme binding; glucocorticoid receptor binding; insulin-like growth factor receptor binding; protein binding; protein domain specific binding; protein heterodimerization activity; sodium channel regulator activity

Biological Process: glucocorticoid catabolic process; glucocorticoid receptor signaling pathway; intracellular protein transport; negative regulation of dendrite morphogenesis; positive regulation of transcription, DNA-dependent; regulation of neuron differentiation; regulation of sodium ion transport; regulation of synaptic plasticity; substantia nigra development