14-3-3 epsilon recombinant protein

14-3-3 epsilon,1-255aa, Human, E Coli

Cat. Num. AAA203051

• Gene Names: YWHAE; MDS; HEL2; MDCR; KCIP-1; 14-3-3E
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: 14-3-3 epsilon; 1-255aa; Human; E Coli; Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein epsilon polypeptide; YWHAE; MDS; MDCR; KCIP-1; FLJ45465; Tyrosine 3/tryptophan 5 -monooxygenase activation protein; epsilon polypeptide; Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein; epsilon polypeptide 14 3 3 E; 14 3 3E; KCIP 1; KCIP1; Mitochondrial import stimulation factor L subunit; Protein kinase C inhibitor protein1; Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide.; 14-3-3 epsilon recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: In 20mM Tris pH7.5
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MDDREDLVYQ AKLAEQAERY DEMVESMKKV AGMDVELTVE ERNLLSVAYK NVIGARRASW RIISSIEQKE ENKGGEDKLK MIREYRQMVE TELKLICCDI LDVLDKHLIP AANTGESKVF YYKMKGDYHR YLAEFATGND RKEAAENSLV AYKAASDIAM TELPPTHPIR LGLALNFSVF YYEILNSPDR ACRLAKAAFD DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQGDGE EQNKEALQDV EDENQ
• Sequence Length: 255

• Applicable Applications for 14-3-3 epsilon recombinant protein: SDS-PAGE
• Antigen Species: Human
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for 14-3-3 epsilon recombinant protein

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon,sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 epsilon, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Recombinant human 14-3-3 epsilon was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for 14-3-3 epsilon recombinant protein

Signal Transduction

References

Oriente F, et al. (2005) J Biol Chem. 280 (49):40642-9.; Conklin D, et al. (1995) PNAS. 92 (17):7892-6;

NCBI and Uniprot Product Information

• NCBI GI #: 5803225
• NCBI GeneID: 7531
• NCBI Accession #: NP_006752
• NCBI GenBank Nucleotide #: NM_006761.4
• UniProt Accession #: P62258; P29360; P42655; Q4VJB6; Q53XZ5; Q63631; Q7M4R4; B3KY71; D3DTH5
• Molecular Weight: 29 kDa (255 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: 14-3-3 protein epsilon
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon
• NCBI Official Symbol: YWHAE
• NCBI Official Synonym Symbols: MDS; HEL2; MDCR; KCIP-1; 14-3-3E
• NCBI Protein Information: 14-3-3 protein epsilon
• UniProt Protein Name: 14-3-3 protein epsilon
• UniProt Gene Name: YWHAE
• UniProt Synonym Gene Names: 14-3-3E

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner (). Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm (PubMed:12917326).