Human urotensin 2 ELISA Kit | UTS2 elisa kit

Human Urotensin II ELISA Kit

Cat. Num. AAA702757

• Gene Names: UTS2; UII; U-II; UCN2; PRO1068
• Reactivity: Human
• Synonyms: urotensin 2; Human Urotensin II ELISA Kit; Human Urotensin II ELISA kit; PRO1068; U-II; UCN2; UII; ; UTS2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human urotensinII. No significant cross-reactivity or interference between human urotensinII and analogues was observed.
• Sequence Length: 124

• Samples: Serum, saliva, cerebrospinal fluid (CSF), tissue homogenates
• Assay Type: Sandwich
• Detection Range: 0.312ng/ml-20ng/ml.
• Sensitivity: The minimum detectable dose of human urotensinIIis typically less than 0.078ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays):CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for UTS2 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for urotensinII has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any urotensinII present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific forurotensinII is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of urotensinII bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 5803209
• NCBI GeneID: 10911
• NCBI Accession #: NP_006777.1
• NCBI GenBank Nucleotide #: NM_006786.3
• UniProt Accession #: O95399; Q5H8X7; Q6UXF6; Q9UKP7
• Molecular Weight: 14,296 Da
• NCBI Official Full Name: urotensin-2 isoform b preproprotein
• NCBI Official Synonym Full Names: urotensin 2
• NCBI Official Symbol: UTS2
• NCBI Official Synonym Symbols: UII; U-II; UCN2; PRO1068
• NCBI Protein Information: urotensin-2; prepro U-II; urotensin II
• UniProt Protein Name: Urotensin-2
• UniProt Gene Name: UTS2
• UniProt Synonym Gene Names: UNQ525/PRO1068; U-II; UII
• UniProt Entry Name: UTS2_HUMAN

NCBI Description

This gene encodes a mature peptide that is an active cyclic heptapeptide absolutely conserved from lamprey to human. The active peptide acts as a vasoconstrictor and is expressed only in brain tissue. Despite the gene family name similarity, this gene is not homologous to urocortin, a member of the sauvagine/corticotropin-releasing factor/urotensin I family. Most of the proprotein is cleaved to make the mature peptide. Transcript variants encoding different preproprotein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Highly potent vasoconstrictor.