Western Blot (WB)
(Western blot analysis of SLAMF3 in 293 cell lysate with SLAMF3 antibody at (A) 1 and (B) 2 μg/mL.)
Rabbit SLAMF3 Polyclonal Antibody | anti-LY9 antibody
SLAMF3 Antibody
Gene Names
LY9; hly9; mLY9; CD229; SLAMF3
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
SLAMF3 Antibody is affinity chromatography purified via peptide column.
Synonyms
SLAMF3; Polyclonal Antibody; SLAMF3 Antibody; hly9; mLY9; CD229; T-lymphocyte surface antigen Ly-9; Cell surface molecule Ly-9; lymphocyte antigen 9; anti-LY9 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
At least three isoforms of SLAMF3 are known to exist. SLAMF3 antibody is predicted to not cross-react with other SLAM protein family members.
Purity/Purification
SLAMF3 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
655
Applicable Applications for anti-LY9 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
SLAMF3 antibody can be used for detection of SLAMF3 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
SLAMF3 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SLAMF3.
Buffer
SLAMF3 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
SLAMF3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of SLAMF3 in 293 cell lysate with SLAMF3 antibody at (A) 1 and (B) 2 μg/mL.)
Western Blot (WB)
(Western blot analysis of SLAMF3 in 293 cell lysate with SLAMF3 antibody at (A) 1 and (B) 2 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of SLAMF3 in 293 cells with SLAMF3 antibody at 10 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of SLAMF3 in 293 cells with SLAMF3 antibody at 10 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of SLAMF3 in 293 cells with SLAMF3 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of SLAMF3 in 293 cells with SLAMF3 antibody at 20 μg/mL.)
Related Product Information for anti-LY9 antibody
SLAMF3 Antibody: The signaling lymphocyte-activation molecule family member 3 (SLAMF3), also known as LY9, is a cell surface receptor that is expressed on T and B lymphocytes and belongs to the CD150/SLAMF1 receptor family. SLAMF3 was identified through a yeast two-hybrid screening in which SLAMF3 bound to the X-linked lymphoproliferative disease gene product SAP, suggesting that in activated T cells, SLAMF3 signaling can be triggered SAP. Its expression on the cell surface appears to be regulated via its interaction with the clathrin-associated adaptor complex 2 (AP-2). Despite its similarity to SLAMF1 in structure and interactions with SAP, SLAMF3-deficient mice do not exhibit phenotypic characteristics associated with SLAMF1- and SAP-deficient mice, suggesting that SLAMF3 plays other roles in T cell activation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
71,961 Da
NCBI Official Full Name
T-lymphocyte surface antigen Ly-9 isoform a
NCBI Official Synonym Full Names
lymphocyte antigen 9
NCBI Official Symbol
LY9
NCBI Official Synonym Symbols
hly9; mLY9; CD229; SLAMF3
NCBI Protein Information
T-lymphocyte surface antigen Ly-9; SLAM family member 3; cell surface molecule Ly-9; signaling lymphocytic activation molecule 3
UniProt Protein Name
T-lymphocyte surface antigen Ly-9
Protein Family
UniProt Gene Name
LY9
UniProt Synonym Gene Names
SLAMF3
UniProt Entry Name
LY9_HUMAN
NCBI Description
LY9 belongs to the SLAM family of immunomodulatory receptors (see SLAMF1; MIM 603492) and interacts with the adaptor molecule SAP (SH2D1A; MIM 300490) (Graham et al., 2006 [PubMed 16365421]).[supplied by OMIM, Mar 2008]
Uniprot Description
CD229: May participate in adhesion reactions between T lymphocytes and accessory cells by homophilic interaction. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Cell surface
Chromosomal Location of Human Ortholog: 1q23.3
Cellular Component: integral to membrane
Molecular Function: receptor activity
Biological Process: cell adhesion
Research Articles on LY9
Similar Products
Product Notes
The LY9 ly9 (Catalog #AAA151301) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SLAMF3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SLAMF3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). SLAMF3 antibody can be used for detection of SLAMF3 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the LY9 ly9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLAMF3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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