Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human [Protein ADP-ribosylarginine] hydrolase (ADPRH) Standard Curve.)
Human [Protein ADP-Ribosylarginine] Hydrolase (ADPRH) ELISA Kit | ADPRH elisa kit
Human [Protein ADP-Ribosylarginine] Hydrolase (ADPRH) ELISA Kit
Gene Names
ADPRH; ARH1; hARH1
Reactivity
Human
Synonyms
[Protein ADP-Ribosylarginine] Hydrolase (ADPRH); Human [Protein ADP-Ribosylarginine] Hydrolase (ADPRH) ELISA Kit; ADPRH; ARH1; ADP-ribose-L-arginine cleaving enzyme; ADPRH elisa kit
Reactivity
Human
Specificity
Human [Protein ADP-ribosylarginine] hydrolase (ADPRH) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADPRH. No significant cross-reactivity or interference between Human ADPRH and analogues was observed.
Sequence Length
357
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human [Protein ADP-ribosylarginine] hydrolase (ADPRH) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human [Protein ADP-ribosylarginine] hydrolase (ADPRH) Standard Curve.)
Related Product Information for ADPRH elisa kit
Description: This Human [Protein ADP-ribosylarginine] hydrolase (ADPRH) ELISA Kit employs a two-site sandwich ELISA to quantitate ADPRH in samples. An antibody specific for ADPRH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADPRH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADPRH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADPRH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The deduced human ADPRH protein has 357 amino acids and an estimated molecular mass of 39.5 kD. Human ADPRH shows 83% amino acid identity to rat Adprh and 82% identity to mouse Adprh. The authors found that the mouse and rat Adprh sequences contain 5 conserved cysteine residues; the human sequence contains only 4 cysteines, with cys108 in rat corresponding to ser103 in human. Expression of recombinant wildtype and mutant rat and human ADPRH proteins in E. coli and assessment of their DTT requirements for hydrolase activity demonstrated that cys108 is responsible for the DTT dependence of rat Adprh. Northern blot analysis detected a 4.1-kb ADPRH transcript in various human tissues, including fetal and adult brain.
Background: The deduced human ADPRH protein has 357 amino acids and an estimated molecular mass of 39.5 kD. Human ADPRH shows 83% amino acid identity to rat Adprh and 82% identity to mouse Adprh. The authors found that the mouse and rat Adprh sequences contain 5 conserved cysteine residues; the human sequence contains only 4 cysteines, with cys108 in rat corresponding to ser103 in human. Expression of recombinant wildtype and mutant rat and human ADPRH proteins in E. coli and assessment of their DTT requirements for hydrolase activity demonstrated that cys108 is responsible for the DTT dependence of rat Adprh. Northern blot analysis detected a 4.1-kb ADPRH transcript in various human tissues, including fetal and adult brain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ADP-ribosylarginine hydrolase isoform 1
NCBI Official Synonym Full Names
ADP-ribosylarginine hydrolase
NCBI Official Symbol
ADPRH
NCBI Official Synonym Symbols
ARH1; hARH1
NCBI Protein Information
ADP-ribosylarginine hydrolase
UniProt Protein Name
[Protein ADP-ribosylarginine] hydrolase
Protein Family
UniProt Gene Name
ADPRH
UniProt Synonym Gene Names
ARH1; ADP-ribosylarginine hydrolase
UniProt Entry Name
ADPRH_HUMAN
NCBI Description
The enzyme encoded by this gene catalyzes removal of mono-ADP-ribose from arginine residues of proteins in the ADP-ribosylation cycle. Unlike the rat and mouse enzymes that require DTT for maximal activity, the human enzyme is DTT-independent. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, May 2014]
Uniprot Description
ADPRH: Catalyzes the reverse reaction of mono-ADP-ribosylation. Belongs to the ADP-ribosylglycohydrolase family.
Protein type: EC 3.2.2.19; Hydrolase
Chromosomal Location of Human Ortholog: 3q13.31-q13.33
Molecular Function: ADP-ribosylarginine hydrolase activity; magnesium ion binding
Biological Process: protein amino acid de-ADP-ribosylation; protein modification process
Research Articles on ADPRH
Similar Products
Product Notes
The Human ADPRH adprh (Catalog #AAA9714611) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714611 ELISA Kit recognizes Human ADPRH. It is sometimes possible for the material contained within the vial of "[Protein ADP-Ribosylarginine] Hydrolase (ADPRH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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