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Western Blot (WB) (Gel: 12%SDS-PAGE Lysate: 40 μ g Lane 1-2: Rat heart tissue and Mouse brain tissue Primary antibody: 1/350 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds)

Rabbit CARTPT Polyclonal Antibody | anti-CARTPT antibody

CARTPT Antibody

Gene Names
CARTPT; CART
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purification
Synonyms
CARTPT; Polyclonal Antibody; CARTPT Antibody; CART; anti-CARTPT antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total CARTPT protein.
Purity/Purification
Antigen Affinity Purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
1.4 mg/ml (varies by lot)
Sequence Length
116
Applicable Applications for anti-CARTPT antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:40-1:250
Immunogen
Full length fusion protein of human CARTPT
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Gel: 12%SDS-PAGE Lysate: 40 μ g Lane 1-2: Rat heart tissue and Mouse brain tissue Primary antibody: 1/350 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds)

Western Blot (WB) (Gel: 12%SDS-PAGE Lysate: 40 μ g Lane 1-2: Rat heart tissue and Mouse brain tissue Primary antibody: 1/350 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using at dilution 1/25,)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using at dilution 1/25,)
Related Product Information for anti-CARTPT antibody
This gene encodes a secreted protein which is processed by prohormone/proprotein convertases to produce smaller, biologically active peptides. Expression of the transcript for this gene is regulated by certain drugs such as cocaine, and the encoded protein is thought to be involved in the regulation of appetite and stress. Mutations in this gene are associated with susceptibility to obesity.
Product Categories/Family for anti-CARTPT antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
12,829 Da
NCBI Official Full Name
cocaine- and amphetamine-regulated transcript protein preproprotein
NCBI Official Synonym Full Names
CART prepropeptide
NCBI Official Symbol
CARTPT
NCBI Official Synonym Symbols
CART
NCBI Protein Information
cocaine- and amphetamine-regulated transcript protein
UniProt Protein Name
Cocaine- and amphetamine-regulated transcript protein
UniProt Gene Name
CARTPT
UniProt Synonym Gene Names
CART
UniProt Entry Name
CART_HUMAN

Uniprot Description

CARTPT: Satiety factor closely associated with the actions of leptin and neuropeptide y; this anorectic peptide inhibits both normal and starvation-induced feeding and completely blocks the feeding response induced by neuropeptide Y and regulated by leptin in the hypothalamus. It promotes neuronal development and survival in vitro. Belongs to the CART family.

Protein type: Hormone; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q13.2

Cellular Component: extracellular space; secretory granule

Molecular Function: neuropeptide hormone activity; protein binding

Biological Process: activation of MAPKK activity; adult feeding behavior; cell glucose homeostasis; cell-cell signaling; cellular response to starvation; circadian regulation of gene expression; G-protein coupled receptor protein signaling pathway; negative regulation of appetite; negative regulation of bone resorption; negative regulation of osteoclast differentiation; neuropeptide signaling pathway; positive regulation of blood pressure; positive regulation of epinephrine secretion; positive regulation of transmission of nerve impulse; signal transduction

Disease: Obesity

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Product Notes

The CARTPT cartpt (Catalog #AAA9415778) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CARTPT Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CARTPT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:500-1:2000 Immunohistochemistry: 1:40-1:250. Researchers should empirically determine the suitability of the CARTPT cartpt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CARTPT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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