Immunocytochemistry (ICC)
(Human alpha-Tubulin is detected by immunocytochemistry using anti-alpha-Tubulin, mAb (rec.)|Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-alphaTubulin, mAb (rec.) (1µg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.|Picture courtesy of Dr. Moutel, Dr. Franck Perez Lab, Curie Institute, Paris.)
Tubulin, alpha Recombinant Protein | TUBA recombinant protein
Tubulin, alpha (Recombinant) (ATTO 488)
Purified from HEK 293 cell culture supernatant (>95% (SDS-PAGE).
Purified from HEK 293 cell culture supernatant (>95% (SDS-PAGE).
Immunocytochemistry (ICC)
(Human alpha-Tubulin is detected by immunocytochemistry using anti-alpha-Tubulin, mAb (rec.)|Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-alphaTubulin, mAb (rec.) (1µg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.|Picture courtesy of Dr. Moutel, Dr. Franck Perez Lab, Curie Institute, Paris.)
Immunocytochemistry (ICC)
(Human alpha-Tubulin is detected by immunocytochemistry using anti-alpha-Tubulin, mAb (rec.)|Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-alphaTubulin, mAb (rec.) (1µg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.|Picture courtesy of Dr. Moutel, Dr. Franck Perez Lab, Curie Institute, Paris.)
Structure
(Structure of the recombinant antibody anti-alpha-Tubulin, mAb (rec.) The single chain variable human fragment (ScFv) selected by antibody phage display technology and specific to the antigen of interest is fused to a human IgG2 Fc region.)
Structure
(Structure of the recombinant antibody anti-alpha-Tubulin, mAb (rec.) The single chain variable human fragment (ScFv) selected by antibody phage display technology and specific to the antigen of interest is fused to a human IgG2 Fc region.)
NCBI and Uniprot Product Information
Uniprot Description
Function: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Subunit structure: Dimer of alpha and beta chains.
Subcellular location: Cytoplasm › cytoskeleton.
Post-translational modification: Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively
By similarity.Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling
By similarity.
Sequence similarities: Belongs to the tubulin family.
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Product Notes
The Tubulin, alpha tuba (Catalog #AAA637918) is a Recombinant Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Tubulin, alpha can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC). Suitable for use in Immunocytochemistry. Researchers should empirically determine the suitability of the Tubulin, alpha tuba for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tubulin, alpha, Monoclonal Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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