1) Make 2 vials of your primary antibody at the working concentration specified on the data sheet or at the best concentration determined by you empirically. 1 vial is for normally processing with your primary antibody, the other vial is for use with the Blocking Peptide.
2) Add the Blocking Peptide at the recommended dilution (see below) into 1 of the vials and incubate at room temperature for 1 hour.
3) Process your tissue/samples as normal with both antibody solutions.
Quick Calculation for Absorption Controls:
When we perform Absoption Controls, we use the peptide at a final concentration of ~10uM (1 x 10-5) or 1uM (1 x 10-6 M). Because most peptides are between 15-20 amino acids with a molecular weight of ~2000 Da, we supply most of our peptide at a concentration of 2mgs/ml. This allows for a simple dilution of 1:100 (~10 uM) or 1:1000 (~1uM) in the working antibody Solution.
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Product Notes
The Delta Opioid Receptor 361-372 (Catalog #AAA555841) is a Blocking Peptide and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: TPSDGPGGGA AA. It is sometimes possible for the material contained within the vial of "Delta Opioid Receptor 361-372, Blocking Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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