Mouse Polyglutamylation Modification Monoclonal Antibody

Polyglutamylation Modification (Polyglutamylated Tubulin, Glutamylated Tubulin, Postranslational Protein Glutamylation)

Cat. Num. AAA642422

• Applications: Western Blot, Immunoprecipitation, Immunocytochemistry
• Purity: Highly Purified; >95% (SDS-PAGE)
• Synonyms: Polyglutamylation Modification; Monoclonal Antibody; Polyglutamylation Modification (Polyglutamylated Tubulin; Glutamylated Tubulin; Postranslational Protein Glutamylation); Anti -Polyglutamylation Modification (Polyglutamylated Tubulin; anti-Polyglutamylation Modification antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: GT335
• Specificity: Recognizes the posttranslational modification (poly)glutamylation on proteins. Reacts with polyglutamylated alpha- and beta-tubulin.
• Purity/Purification: Highly Purified; >95% (SDS-PAGE)
• Form/Format: Supplied as a liquid in PBS, 10% glycerol, 0.02% sodium azide.

• Applicable Applications for anti-Polyglutamylation Modification antibody: Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Immunocytochemistry, Immunoprecipitation, Western Blot
• Immunogen: Octapeptide EGEGE*EEG, modified by the addition of two glutamyl units onto the fifth E (indicated by an asterisk).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunofluorescence (IF)

(Immunofluorescence analysis of tubulin glutamylation with MBS642422. HeLa cells grown in standard culture conditions are transfected with GFP-fusion expression plasmids for the polyglutamylases TTLL4 and TTLL6. Glutamylated microtubules are detected with MBS642422. Only in the cells expressing glutamylases (red arrowheads), strong microtubule labelling is detected, whereas in non-transfected cells (white arrowheads), only very weak labelling is seen. Note that the level of endogenous microtubule glutamylation can vary between different cell lines. Picture courtesy of Dr. Carsten Janke, Curie Institute, Paris.)

Western Blot (WB)

(Western blot analysis of protein glutamylation with MBS642422 to polyglutamylation modification .|Method: HeLa cells grown in standard culture conditions are lysed in SDS sample buffer and run on 10% SDS-PAGE. The proteins are transferred to nitrocellulose membrane and detected by standard Western blot protocol, using the MAb to polyglutamylation modification (1:1’000) in TBS containing 0.1% Tween 20 for washing steps and 5% fat free milk for antibody incubation.is)

Testing Data

(In HeLa cells, only two non-tubulin proteins are detected (Regnard et al., 2000; van Dijk et al., 2008), whereas after the expression of either TTLL4 or TTLL6 polyglutamylases (van Dijk et al., 2007), tubulin and additional proteins are detected. Brain tubulin is highly polyglutamylated and therefore strongly detected with the 045161|Picture courtesy of Dr Carsten Janke, Curie Institute, Par)

Related Product Information for anti-Polyglutamylation Modification antibody

Polyglutamylation is a post-translational modification in which glutamate side chains of variable lengths are formed on the modified protein. It is evolutionarily conserved from and the most prominent substrate is tubulin, the microtubule (MT) building block. Polyglutamylation has been proposed to be involved in the functional adaptation of MTs, as it occurs within the carboxy-terminal tubulin tails that participate directly in the binding of many structural and motor MT-associated proteins. The recent identification of new substrates of polyglutamylation indicates that this post-translational modification could be a potential regulator of diverse cellular processes and involved in cell cycle and cell proliferation.

Product Categories/Family for anti-Polyglutamylation Modification antibody

Antibodies; Abs to Tubulin