Testing Data
(Schematic antibody structure.)
Tubulin, beta Recombinant Protein | TUBB recombinant protein
Tubulin, beta (Recombinant) (Tubulin beta Chain, Tubulin beta-5 chain)
Purified from HEK 293 cell culture supernatant (>95% (SDS-PAGE).
Purified from HEK 293 cell culture supernatant (>95% (SDS-PAGE).
Testing Data
(Schematic antibody structure.)
Testing Data
(Schematic antibody structure.)
Immunocytochemistry (ICC)
(Human β-Tubulin is detected by immunocytochemistry using MBS636051.|Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-beta-Tubulin, mAb (rec.) (1µg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.|Picture courtesy of Dr. Moutel, Dr. Franck Perez lab, Curie Institute, Paris.)
Immunocytochemistry (ICC)
(Human β-Tubulin is detected by immunocytochemistry using MBS636051.|Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-beta-Tubulin, mAb (rec.) (1µg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.|Picture courtesy of Dr. Moutel, Dr. Franck Perez lab, Curie Institute, Paris.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014]
Uniprot Description
TUBB: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. May interact with RNABP10. Interacts with PIFO/C1orf88. Ubiquitously expressed with highest levels in spleen, thymus and immature brain. Belongs to the tubulin family.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 6p21.33
Cellular Component: microtubule; cytoskeleton; nuclear envelope lumen; nucleus; cytosol
Molecular Function: GTPase activity; protein domain specific binding; protein binding; GTP binding; structural constituent of cytoskeleton; MHC class I protein binding; ubiquitin protein ligase binding; protein complex binding; GTPase activating protein binding; structural molecule activity
Biological Process: protein polymerization; natural killer cell mediated cytotoxicity; metabolic process; cell division; organelle organization and biogenesis; mitotic cell cycle; cytoskeleton-dependent intracellular transport; spindle assembly; cell motility; G2/M transition of mitotic cell cycle; cellular process; microtubule-based process
Disease: Cortical Dysplasia, Complex, With Other Brain Malformations 6
Research Articles on TUBB
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Product Notes
The TUBB tubb (Catalog #AAA636051) is a Recombinant Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Tubulin, beta can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC). Suitable for use in ELISA, Immunocytochemistry, Western Blot. Researchers should empirically determine the suitability of the TUBB tubb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tubulin, beta, Monoclonal Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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