Rab1-GTP Recombinant Protein

Rab1-GTP (Recombinant) (Ras-related Protein Rab-1)

Cat. Num. AAA636467

• Applications: Immunoprecipitation, Immunocytochemistry
• Purity: Highly Purified; Purified from HEK 293 cell culture supernatant (>95% (SDS-PAGE).
• Synonyms: Rab1-GTP; Monoclonal Recombinant Protein; Rab1-GTP (Recombinant) (Ras-related Protein Rab-1); Anti -Rab1-GTP (Recombinant) (Ras-related Protein Rab-1); Rab1-GTP recombinant protein

• Host: Human
• Clonality: Monoclonal
• Isotype: IgG2,l
• Clone Number: ROF7
• Specificity: Recognizes human, mouse, rat and canine Rab1a-GTP and Rab1b-GTP.
• Purity/Purification: Highly Purified; Purified from HEK 293 cell culture supernatant (>95% (SDS-PAGE).
• Form/Format: Supplied as a liquid in PBS, 10% glycerol, 0.02% sodium azide.

• Applicable Applications for Rab1-GTP recombinant protein: Immunoprecipitation (IP), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Immunocytochemistry, Immunoprecipitation
• Immunogen: Full length canine Rab1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Schematic antibody structure.)

Immunocytochemistry (ICC)

(Rab1-GTP is detected by immunocytochemistry using anti-Rab1-GTP, mAb.|Method: HeLa cells are grown in standard culture conditions, fixed with paraformaldehyde (3%), permeablized in PBS+ BSA 0.2 % + Saponin 0.05 % and incubated with anti-Rab1-GTP, mAb (1µg /ml ) in PBS-BSA-Saponin). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody in PBS-BSA-Saponin for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.|Picture courtesy of Dr. Moutel, Dr. Franck Perez lab, Curie Institute, Paris.)

Related Product Information for Rab1-GTP recombinant protein

Rab1 (Ypt1 in yeast) is a small GTPase that plays a well-established role in mediating ER-to-Golgi protein transport in both yeast and mammalian cells. Rab1 recruits effector proteins to budding COPII vesicles at the ER, forming cis-SNARE complexes that promote targeting to and fusion of these vesicles with the cis-Golgi. Rab1 is also involved in COPI vesicle formation and other distinct transport pathways, including ER-to-Golgi intermediate compartment (ERGIC)-to-cell periphery trafficking. Recently Rab1 has been shown to function in antimicrobial autophagy (autophagosome formation), as well as other forms of autophagy in mammalian cells in a way independent of ER-to-Golgi trafficking. anti-Rab1-GTP, monoclonal antibody (recombinant) (ROF7) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Product Categories/Family for Rab1-GTP recombinant protein

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins