Immunocytochemistry (ICC)
(Human giantin is detected by immunocytochemistry using anti-Giantin, mAb (rec.)|Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with using anti-Giantin, mAb (rec.) 1μg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are mounted in Moewiol. Nuclei are stained with DAPI.|Picture courtesy of Dr. Moutel, Dr. Franck Perez Lab, Curie Institute, Paris.)
Giantin Recombinant Protein | GOLGB1 recombinant protein
Giantin (Recombinant) (ATTO 488) (Golgin Subfamily B Member 1, 372kD Golgi Complex-associated Protein, GCP372, Macrogolgin)
Gene Names
GOLGB1; GCP; GCP372; GOLIM1
Applications
Immunocytochemistry
Purity
Highly PurifiedPurified from HEK 293 cell culture supernatant (>95% (SDS-PAGE).
Synonyms
Giantin; Monoclonal Recombinant Protein; Giantin (Recombinant) (ATTO 488) (Golgin Subfamily B Member 1; 372kD Golgi Complex-associated Protein; GCP372; Macrogolgin); Anti -Giantin (Recombinant) (ATTO 488) (Golgin Subfamily B Member 1; GOLGB1 recombinant protein
Host
Human
Clonality
Monoclonal
Isotype
IgG2,l
Clone Number
TA10
Specificity
Recognizes human and mouse giantin.
Purity/Purification
Highly Purified
Purified from HEK 293 cell culture supernatant (>95% (SDS-PAGE).
Purified from HEK 293 cell culture supernatant (>95% (SDS-PAGE).
Form/Format
Supplied as a liquid in PBS, 0.02% sodium azide.
Applicable Applications for GOLGB1 recombinant protein
Immunocytochemistry (ICC)
Application Notes
Suitable for use in Immunocytochemistry
Immunogen
Human recombinant giantin.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunocytochemistry (ICC)
(Human giantin is detected by immunocytochemistry using anti-Giantin, mAb (rec.)|Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with using anti-Giantin, mAb (rec.) 1μg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are mounted in Moewiol. Nuclei are stained with DAPI.|Picture courtesy of Dr. Moutel, Dr. Franck Perez Lab, Curie Institute, Paris.)
Immunocytochemistry (ICC)
(Human giantin is detected by immunocytochemistry using anti-Giantin, mAb (rec.)|Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with using anti-Giantin, mAb (rec.) 1μg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are mounted in Moewiol. Nuclei are stained with DAPI.|Picture courtesy of Dr. Moutel, Dr. Franck Perez Lab, Curie Institute, Paris.)
Structure
(Structure of the recombinant antibody anti-Giantin (h), mAb (rec.)The single chain variable human fragment (ScFv) selected by antibody phage display technology and specific to the antigen of interest is fused to a human IgG2 Fc region.)
Structure
(Structure of the recombinant antibody anti-Giantin (h), mAb (rec.)The single chain variable human fragment (ScFv) selected by antibody phage display technology and specific to the antigen of interest is fused to a human IgG2 Fc region.)
Related Product Information for GOLGB1 recombinant protein
Giantin, the largest Golgi membrane protein, is C-terminally anchored to cis- and medial-Golgi membranes and forms a long ~250nm coiled-coil rod that directly interacts with microtubules in a cell-cycle regulated way. It is an excellent marker for mammalian golgi complex. It is also an antigen in chronic rheumatoid arthritis and in the autoimmune disease Sjoegren syndrome.
Product Categories/Family for GOLGB1 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
376,019 Da
NCBI Official Full Name
Golgin subfamily B member 1 isoform 4
NCBI Official Synonym Full Names
golgin B1
NCBI Official Symbol
GOLGB1
NCBI Official Synonym Symbols
GCP; GCP372; GOLIM1
NCBI Protein Information
Golgin subfamily B member 1; giantin; macrogolgin; golgi integral membrane protein 1; 372 kDa Golgi complex-associated protein; golgin B1, golgi integral membrane protein; golgi autoantigen, golgin subfamily b, macrogolgin (with transmembrane signal), 1
UniProt Protein Name
Golgin subfamily B member 1
Protein Family
UniProt Gene Name
GOLGB1
UniProt Synonym Gene Names
GCP372
UniProt Entry Name
GOGB1_HUMAN
Uniprot Description
GOLGB1: May participate in forming intercisternal cross-bridges of the Golgi complex.
Protein type: Membrane protein, integral; Vesicle
Chromosomal Location of Human Ortholog: 3q13
Cellular Component: Golgi membrane; Golgi apparatus; Golgi stack; cis-Golgi network; membrane; integral to membrane; ER-Golgi intermediate compartment
Molecular Function: protein binding
Biological Process: Golgi organization and biogenesis
Research Articles on GOLGB1
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Product Notes
The GOLGB1 golgb1 (Catalog #AAA636133) is a Recombinant Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Giantin can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC). Suitable for use in Immunocytochemistry. Researchers should empirically determine the suitability of the GOLGB1 golgb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Giantin, Monoclonal Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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