Reactivity Chart
IgG secondary antibody
Anti-Goat IgG, HRP
Reactivity
Goat
Applications
Western Blot, ELISA
Purity
Epitope affinity purified
Synonyms
IgG; Polyclonal Secondary Antibody; Anti-Goat IgG; HRP; Immunoglobulin Y antibody; IgG secondary antibody
Host
Chicken
Reactivity
Goat
Clonality
Polyclonal
Isotype
IgY
Specificity
Using serum samples detects goat IgG by Western blot. Reduced cross-reactivity to other species.
Purity/Purification
Epitope affinity purified
Form/Format
Polyclonal antibody supplied as a 500ul (2mg/ml) aliquot in PBS, 50% glycerol and 0.05% sodium azide.
Concentration
2mg/ml (varies by lot)
Applicable Applications for IgG secondary antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:2,000-1:10,000
ELISA:20,000-100,000
ELISA:20,000-100,000
Conjugation
HRP
Immunogen
Full goat IgG affinity purified from domestic goat (Capra hircus) serum using protein G. Purity >95% by SDS-PAGE.
Preparation and Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Reactivity Chart
Reactivity Chart
Western Blot (WB)
(Western blot using using lysates at 30 ug per lane; 1st AbAnti-GAPDH Ab (AB0049) at 1:2,500 and 2nd AbAnti-goat conjugated to HRP (AB1125) at 1:10,000;)
Western Blot (WB)
(Western blot using using lysates at 30 ug per lane; 1st AbAnti-GAPDH Ab (AB0049) at 1:2,500 and 2nd AbAnti-goat conjugated to HRP (AB1125) at 1:10,000;)
Testing Data
(Anti-HSP20 Ab at 1:2,000 dilution; 30 ng of protein per lane; chicken polyclonal to goat IgG conjugated to HRP (AB1125) at 1/10,000 dilution;)
Testing Data
(Anti-HSP20 Ab at 1:2,000 dilution; 30 ng of protein per lane; chicken polyclonal to goat IgG conjugated to HRP (AB1125) at 1/10,000 dilution;)
Related Product Information for IgG secondary antibody
Chicken polyclonal antibody to goat IgG conjugated to horseradish peroxidase (HRP).
Similar Products
Product Notes
The IgG (Catalog #AAA448216) is a Secondary Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The Anti-Goat IgG, HRP reacts with Goat and may cross-react with other species as described in the data sheet. AAA Biotech's IgG can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:2,000-1:10,000 ELISA:20,000-100,000. Researchers should empirically determine the suitability of the IgG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG, Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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