TREM-1 ELISA Pair Set | TREM1 elisa pair set

TREM-1 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118266

• Synonyms: TREM-1; TREM-1 Matched ELISA Antibody Pair Set; Human; Human TREM-1 ELISA Pair Set; triggering receptor expressed on myeloid cells 1; CD354 Matched ELISA Antibody Pair Set; TREM1 elisa pair set

• Specificity: Quantitative determination of Human TREM-1

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 23.44-1500pg/mL
• Sensitivity: 23.44pg/ml
• Materials Provided: 1. Capture Antibody 1 mg/mL of mouse anti-Human TREM-1 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of mouse anti-Human TREM-1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in Dilution Buffer before use. ; 3. Standard Each vial contains 245 ng of recombinant Human TREM-1. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 1500 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for TREM1 elisa pair set

TREM1 (triggering receptor expressed on myeloid cells) is a type I transmembrane protein with a single Ig-like domain, and is selectively expressed on blood neutrophils and a subset of monocytes. As a member of the growing family of receptors related to NK cell receptors, TREM1 activates downstream signaling events with the help of an adapter protein called DAP12. Expression of TREM1 is up-regulated by bacterial LPS, a ligand for TLR4, as well as lipoteichoic acid. Although its natural ligand has not been identified, engagement of TREM1 with agonist mAbs triggers secretion of the proinflammatory cytokines TNF-alpha and IL-1beta, as well as chemokines such as IL-8 and monocyte chemoattractant protein (MCP)-1. Intracellularly, TREM1 induces Ca2+ mobilization and tyrosine phosphorylation of extracellular signal-related kinase 1 (ERK1), ERK2 and phospholipase C-gamma. In an animal model of LPS-induced septic shock, blockade of TREM1 signaling inhibited hyperresponsiveness and death. Thus, it has been demonstrated that TREM1 performs a critical function in immune responses involved in host defense against microbial challenges, and is suggested to be a potential therapeutic target for septic shock.

This TREM-1 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human TREM-1. It contains Human TREM-1 capture antibody, Human TREM-1 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human TREM-1 protein.

References

Bouchon, A. et al., 2000, J. Immunol. 164: 4991-4995. Bouchon, A. et al., 2001, Nature. 410: 1103-1107. Bleharski, J.R. et al., 2003, J. Immunol. 170: 3812-3818.