DC-SIGN ELISA Pair Set | CD209 elisa pair set

DC-SIGN Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118210

• Synonyms: DC-SIGN; DC-SIGN Matched ELISA Antibody Pair Set; Human; Human DC-SIGN/CD209 ELISA Pair Set; CD209 molecule; CD209 Matched ELISA Antibody Pair Set; CDSIGN Matched ELISA Antibody Pair Set; CLEC4L Matched ELISA Antibody Pair Set; DC-SIGN1 Matched ELISA Antibody Pair Set; MGC129965 Matched ELISA Antibody Pair Set; CD209 elisa pair set

• Specificity: Quantitative determination of Human DC-SIGN

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 23.44-1500pg/mL
• Sensitivity: 23.4375pg/ml
• Materials Provided: 1. Capture Antibody 0.2 mg/mL of mouse anti-CD209 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL rabbit anti-CD209 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 165 ng of recombinant CD209. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1.5ng/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for CD209 elisa pair set

Dendritic cell (DC)-specific intercellular adhesion molecule 3 (ICAM-3) grabbing nonintegrin (DC-SIGN), also known as CD209, is a type II transmembrane protein on DCs with a C-type lectin extracellular domain, is capable of binding ICAM-3 on resting T cells in the secondary lymphoid organs, providing the initial contact between these cells during the establishment of cell-mediated immunity. It is not only a pattern recognition receptor but implicated in immunoregulation of DCs. It has an important role in mediating DC adhesion, migration, inflammation, activating primary T cell, triggering immune response and participating in immune escape of pathogens and tumors. DC-SIGN also mediates the capture and internalization of viral, bacterial, and fungal pathogens by dendritic cells, such as HIV-1, Ebola virus, cytomegalovirus, Dengue virus, and hepatitis C virus. DC-SIGN is unique in that it regulates adhesion processes, such as DC trafficking and T-cell synapse formation, as well as antigen capture. Moreover, even though several C-type lectins have been shown to bind HIV-1, DC-SIGN does not only capture HIV-1 but also protects it in early endosomes allowing HIV-1 transport by DC to lymphoid tissues, where it enhances trans infection of T cells.

This DC-SIGN Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human DC-SIGN. It contains Human DC-SIGN capture antibody, Human DC-SIGN detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human DC-SIGN protein.

References

Geijtenbeek TB, et al. (2002) DC-SIGN, a C-type lectin on dendritic cells that unveils many aspects of dendritic cell biology. J Leukoc Biol. 71(6): 921-31.
Masso M. (2003) DC-SIGN points the way to a novel mechanism for HIV-1 transmission. MedGenMed. 5(2): 2.
Zhou T, et al. (2006) DC-SIGN and immunoregulation. Cell Mol Immunol. 3(4): 279-83.