Testing Data
ST2/IL-1 RL1 ELISA Pair Set | IL1RL1 elisa pair set
ST2/IL-1 RL1 Matched ELISA Antibody Pair Set, Human
Synonyms
ST2/IL-1 RL1; ST2/IL-1 RL1 Matched ELISA Antibody Pair Set; Human; Human IL1RL1/DER4 ELISA Pair Set; interleukin 1 receptor-like 1; DER4 Matched ELISA Antibody Pair Set; FIT-1 Matched ELISA Antibody Pair Set; IL1RL1 Matched ELISA Antibody Pair Set; IL33R Matched ELISA Antibody Pair Set; IL-1 R4 Matched ELISA Antibody Pair Set; ST2 Matched ELISA Antibody Pair Set; ST2L Matched ELISA Antibody Pair Set; ST2V Matched ELISA Antibody Pair Set; T1 Matched ELISA Antibody Pair Set; IL1RL1 elisa pair set
Specificity
Quantitative determination of Human ST2/IL-1 RL1
Assay Type
Solid Phase Sandwich ELISA
Detection Range
7.8-500pg/mL
Sensitivity
7.8pg/ml
Materials Provided
1. Capture Antibody 1 mg/mL of mouse anti-Human IL1RL1/DER4 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating.
2. Detection Antibody 0.2 mg/mL of mouse anti-Human IL1RL1/DER4 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 1.2ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 28 ng of recombinant Human IL1RL1/DER4. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 500 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of mouse anti-Human IL1RL1/DER4 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 1.2ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 28 ng of recombinant Human IL1RL1/DER4. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 500 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for IL1RL1 elisa pair set
IL-1 receptor-like 1 (IL1RL1) is a membrane receptor involved in TH2 inflammatory responses and eosinophilia. It has previously been described that levels of the interleukin-1 like 1 (IL1RL1) protein can be used to identifies cardiovascular disease and determine the prognosis for a patient with cardiovascular disease. The ligand for IL1RL1 has been described and named IL-33. Mutants in IL1RL1 have been associated with blood eosinophil counts in a genome-wide association study and with asthma in family-based and case-control studies. As an important mediator involved in many immune and inflammatory responses, this cytokine has been implicated as a regulator of both the development and effector phases of type 2 helper T cell responses, and as a negative feedback modulator of macrophage proinflammatory function. IL33 is a specific ligand of ST2L and induces the production of Th2 cytokines.
This ST2/IL-1 RL1 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human ST2/IL-1 RL1. It contains Human ST2/IL-1 RL1 capture antibody, Human ST2/IL-1 RL1 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human ST2/IL-1 RL1 protein.
This ST2/IL-1 RL1 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human ST2/IL-1 RL1. It contains Human ST2/IL-1 RL1 capture antibody, Human ST2/IL-1 RL1 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human ST2/IL-1 RL1 protein.
Product Categories/Family for IL1RL1 elisa pair set
References
Savenije OE, et al. (2011) Interleukin-1 receptor-like 1 polymorphisms are associated with serum IL1RL1-a, eosinophils, and asthma in childhood. J Allergy Clin Immunol. 127(3): 750-6.Li H, et al. (2000) The cloning and nucleotide sequence of human ST2L cDNA. Genomics. 67(3): 284-90.
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Product Notes
The IL1RL1 (Catalog #AAA8118185) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "ST2/IL-1 RL1, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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