M-CSF ELISA Pair Set | CSF1 elisa pair set

M-CSF Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118404

• Synonyms: M-CSF; M-CSF Matched ELISA Antibody Pair Set; Human; Human M-CSF/CSF-1 ELISA Pair Set; colony stimulating factor 1 (macrophage); CSF-1 Matched ELISA Antibody Pair Set; MCSF Matched ELISA Antibody Pair Set; CSF1 elisa pair set

• Specificity: Quantitative determination of Human M-CSF

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 23.44-1500pg/mL
• Sensitivity: 23.44pg/ml
• Materials Provided: 1. Capture Antibody 0.5 mg/mL of rabbit anti-Human M-CSF/CSF-1 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of rabbit anti-Human M-CSF/CSF-1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 22 ng of recombinant Human M-CSF/CSF-1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1500 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for CSF1 elisa pair set

Macrophage colony-stimulating factor 1, also known as CSF-1, M-CSF, Lanimostim and CSF1, is a single-pass membrane protein which is disulfide-linked as a homodimer or heterodimer. Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. M-CSF/CSF-1 is known to facilitate monocyte survival, monocyte-to-macrophage conversion, and macrophage proliferation. M-CSF/CSF-1 is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. It binds to the Colony stimulating factor 1 receptor. M-CSF/CSF-1 may also be involved in development of the placenta. The active form of M-CSF/CSF-1 is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. M-CSF/CSF-1 induces cells of the monocyte/macrophage lineage. It also plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Upregulation of M-CSF/CSF-1 in the infarcted myocardium may have an active role in healing not only through its effects on cells of monocyte/macrophage lineage, but also by regulating endothelial cell chemokine expression.

This M-CSF Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human M-CSF. It contains Human M-CSF capture antibody, Human M-CSF detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human M-CSF protein.

References

Pandit J. et al., 1992, Science. 258: 1358-62. Tokai M. et al., 2000, J Bacteriol. 182 (10): 2865-8. Fan X. et al., 2001, Am J Physiol Endocrinol Metab. 280 (1): E103-11. Frangogiannis NG. et al., 2003, Am J Physiol Heart Circ Physiol. 285 (2): H483-92. Cupp JS. et al., 2007, Am J Surg Pathol. 31 (6): 970-6.