IL22 elisa pair set

IL22 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118440

• Synonyms: IL22; IL22 Matched ELISA Antibody Pair Set; Human; Human IL22 ELISA Pair Set; interleukin 22; IL-22 Matched ELISA Antibody Pair Set; IL-D110 Matched ELISA Antibody Pair Set; IL-TIF Matched ELISA Antibody Pair Set; ILTIF Matched ELISA Antibody Pair Set; TIFa Matched ELISA Antibody Pair Set; TIFIL-23 Matched ELISA Antibody Pair Set; zcyto18 Matched ELISA Antibody Pair Set; IL22 elisa pair set

• Specificity: Quantitative determination of Human IL22

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 2.03-130pg/mL
• Sensitivity: 2.03pg/ml
• Materials Provided: 1. Capture Antibody 1 mg/mL of mouse anti-Human IL22 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of rabbit anti-Human IL22 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 8.8 ng of recombinant Human IL22. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 130 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for IL22 elisa pair set

IL22 is a member of a group of cytokines called the IL-1 family of IL-1 superfamily (including IL-19, IL-2, IL-24, and IL-26), a class of potent mediators of cellular inflammatory responses. It shares the use of IL-1R2 in cell signaling with other members of this family, IL-1, IL-26, IL-28A/B, and IL-29. IL22 is produced by activated DC and T cells and initiates innate immune responses against bacterial pathogens especially in epithelial cells such as respiratory and gut epithelial cells. IL22 along with IL-17 is rapidly produced by splenic LTi-like cells and can be also produced by Th17 cells and likely plays a role in the coordinated response of both adaptive and innate immune systems. IL22 biological activity is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-1R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (SIL-22R1). IL22 and IL-1 receptor chains play a role in cellular targeting and signal transduction to selectively initiate and regulate immune responses. IL22 can contribute to immune disease through the stimulation of inflammatory responses, S1s, and defensins. IL22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-1. In some contexts, the pro-inflammatory versus tissue-protective functions of IL22 are regulated by the often co-expressed cytokine IL-17A.

This IL22 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human IL22. It contains Human IL22 capture antibody, Human IL22 detector antibody (HRP) and a highly purified recombinant Human IL22 protein.

References

Pestka S. et al., 2004, Annu Rev Immunol. 22: 929-79. Xie MH. et al., 2000, J Biol Chem. 275 (40): 31335-9. Jones BC. et al., 2008, Structure. 16 (9): 1333-44.