SDS-Page
TNFRSF1A Associated Via Death Domain Protein (TRADD) Recombinant Protein | TRADD recombinant protein
Recombinant TNFRSF1A Associated Via Death Domain Protein (TRADD)
Gene Names
Tradd; AA930854; 9130005N23Rik
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
TNFRSF1A Associated Via Death Domain Protein (TRADD); Recombinant TNFRSF1A Associated Via Death Domain Protein (TRADD); TRADD recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-SAVDKVILSE AYTDPKKKVA IYKALQTALS ESGDSSDVLQ ILKIHCSDPQ LIVQLRFCGR VLCGRFLQAY REGALRTALQ RCMAPALAQE ALRLQLELRA GAEQLDSWLT DEERCLNYIL AQKPDRLRDE ELAELEDELC KLTCDCTGQG GAIQVASAGS KFPVSSPTEE KPLPAACQTF LFHGQLVVNR PLTLQDQQTF ARSVGLKWRR VGRSLQRNCR ALRDPALDSL AYEYERDGL
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-SAVDKVILSE AYTDPKKKVA IYKALQTALS ESGDSSDVLQ ILKIHCSDPQ LIVQLRFCGR VLCGRFLQAY REGALRTALQ RCMAPALAQE ALRLQLELRA GAEQLDSWLT DEERCLNYIL AQKPDRLRDE ELAELEDELC KLTCDCTGQG GAIQVASAGS KFPVSSPTEE KPLPAACQTF LFHGQLVVNR PLTLQDQQTF ARSVGLKWRR VGRSLQRNCR ALRDPALDSL AYEYERDGL
Sequence Length
310
Applicable Applications for TRADD recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Mus musculus (Mouse)
Expression System
Prokaryotic expression
Residues
Ser21~Leu259 (Accession # Q3U0V2) with two N-terminal Tags, His-tag and T7-tag
Subcellular Location
Nucleus. Cytoplasm. Cytoplasm, cytoskeleton
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Predicted Isoelectric Point
7.1
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30.5kDa
NCBI Official Full Name
tumor necrosis factor receptor type 1-associated DEATH domain protein
NCBI Official Synonym Full Names
TNFRSF1A-associated via death domain
NCBI Official Symbol
Tradd
NCBI Official Synonym Symbols
AA930854; 9130005N23Rik
NCBI Protein Information
tumor necrosis factor receptor type 1-associated DEATH domain protein; TNFR1-associated DEATH domain protein; TNF receptor 1 associated signal transducer
UniProt Protein Name
Tumor necrosis factor receptor type 1-associated DEATH domain protein
UniProt Gene Name
Tradd
UniProt Synonym Gene Names
TNFR1-associated DEATH domain protein
UniProt Entry Name
TRADD_MOUSE
Uniprot Description
TRADD: Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B. Heterodimer with TNFRSF1A/TNFR1. Interacts with DAB2IP, FADD, HIPK2, KRT14, KRT16, KRT17, KRT18, RIPK1, SQSTM1, TRAF1, TRAF2 and TRPC4AP. Found in all examined tissues.
Protein type: Apoptosis; Adaptor/scaffold
Cellular Component: cytoskeleton; protein complex; cytoplasm; nucleus; receptor complex; lipid raft
Molecular Function: identical protein binding; protein binding; signal transducer activity; protein complex binding; tumor necrosis factor receptor binding; kinase binding; molecular adaptor activity
Biological Process: positive regulation of hair follicle development; regulation of apoptosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein heterooligomerization; apoptosis; signal transduction
Research Articles on TRADD
Similar Products
Product Notes
The TRADD tradd (Catalog #AAA2009170) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TNFRSF1A Associated Via Death Domain Protein (TRADD) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the TRADD tradd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-SAV DKVILSE AYTDPKKKVA IYKALQTALS ESGDSSDVLQ ILKIHCSDPQ LIVQLRFCGR VLCGRFLQAY REGALRTALQ RCMAPALAQE ALRLQLELRA GAEQLDSWLT DEERCLNYIL AQKPDRLRDE ELAELEDELC KLTCDCTGQG GAIQVASAGS KFPVSSPTEE KPLPAACQTF LFHGQLVVNR PLTLQDQQTF ARSVGLKWRR VGRSLQRNCR ALRDPALDSL AYEYERDGL. It is sometimes possible for the material contained within the vial of "TNFRSF1A Associated Via Death Domain Protein (TRADD), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
