Sequence Information
Interleukin 6 (IL6) Recombinant Protein | IL6 recombinant protein
Recombinant Interleukin 6 (IL6)
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Interleukin 6 (IL6); Recombinant Interleukin 6 (IL6); IL6 recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with a N-terminal His-tag, its sequence is listed below.
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGS-PE RLEEDAKGDA TSDKMLFTSP DKTEELIKYI LGKISAMRKE MCEKYEKCEN SKEVLAENNL NLPKMAEKDG CFQSGFNQET CLMRITTGLV EFQIYLDYLQ KEYESNKGNV EAVQISTKAL IQTLRQKGKN PDKATTPNPT TNAGLLDKLQ SQNEWMKNTK IILILRSLED FLQFSLRAIR IM
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGS-PE RLEEDAKGDA TSDKMLFTSP DKTEELIKYI LGKISAMRKE MCEKYEKCEN SKEVLAENNL NLPKMAEKDG CFQSGFNQET CLMRITTGLV EFQIYLDYLQ KEYESNKGNV EAVQISTKAL IQTLRQKGKN PDKATTPNPT TNAGLLDKLQ SQNEWMKNTK IILILRSLED FLQFSLRAIR IM
Sequence Length
212
Applicable Applications for IL6 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Sus scrofa; Porcine (Pig)
Expression System
Prokaryotic expression
Residues
Pro29~Met212 (Accession # P26893) with a N-terminal His-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Sequence Information
Sequence Information
SDS-Page
SDS-Page
Testing Data
Testing Data
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24.6kDa
NCBI Official Full Name
interleukin-6
NCBI Official Symbol
IL-6
NCBI Protein Information
interleukin-6; interleukin 6
UniProt Protein Name
Interleukin-6
Protein Family
UniProt Gene Name
IL6
UniProt Synonym Gene Names
IL-6
UniProt Entry Name
IL6_PIG
Uniprot Description
Function: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance
By similarity.
Subcellular location: Secreted.
Sequence similarities: Belongs to the IL-6 superfamily.
Research Articles on IL6
Similar Products
Product Notes
The IL6 il6 (Catalog #AAA2010845) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Interleukin 6 (IL6) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the IL6 il6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with a N-terminal His-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS-PE RLEEDAKGDA TSDKMLFTSP DKTEELIKYI LGKISAMRKE MCEKYEKCEN SKEVLAENNL NLPKMAEKDG CFQSGFNQET CLMRITTGLV EFQIYLDYLQ KEYESNKGNV EAVQISTKAL IQTLRQKGKN PDKATTPNPT TNAGLLDKLQ SQNEWMKNTK IILILRSLED FLQFSLRAIR IM. It is sometimes possible for the material contained within the vial of "Interleukin 6 (IL6), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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