Growth Hormone Receptor (GHR) Recombinant Protein | GHR recombinant protein

Recombinant Growth Hormone Receptor (GHR)

Cat. Num. AAA2018770

• Gene Names: Ghr; GHBP; GHR/BP
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Growth Hormone Receptor (GHR); Recombinant Growth Hormone Receptor (GHR); GHR recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LQRIN PSLGTSSSGK PRFTKCRSPE LETFSCYWTE GDNPDLKTPG SIQLYYAKRE SQRQAARIAH EWTQEWKECP DYVSAGKNSC YFNSSYTSIW IPYCIKLTTN GDLLDQKCFT VDEIVQPDPP IGLNWTLLNI SLTGIRGDIQ VSWQPPPNAD VLKGWIILEY EIQYKEVNES KWKVMGPIWL TYCPVYSLRMDKEHEVRVRS RQRSFEKYSE FSEVLRVIFP QTNILE
• Sequence Length: 297

• Applicable Applications for GHR recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Leu36~Glu266 (Accession # P16882) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Cell membrane; Single-pass type I membrane protein. Secreted
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 7.1
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 114520605
• NCBI GeneID: 14600
• NCBI Accession #: NP_001041643.1
• NCBI GenBank Nucleotide #: NM_001048178.2
• UniProt Accession #: P16882; P16590; Q61653; Q6DI66; Q80W86; Q8R1M5; Q920Z3; Q9R264
• Molecular Weight: 30.7kDa
• NCBI Official Full Name: growth hormone receptor isoform 2
• NCBI Official Synonym Full Names: growth hormone receptor
• NCBI Official Symbol: Ghr
• NCBI Official Synonym Symbols: GHBP; GHR/BP
• NCBI Protein Information: growth hormone receptor; GH receptor; somatotropin receptor; growth hormone receptor/binding protein; Growth hormone receptor precursor (GH receptor) (GH binding protein) (GHBP) (Serum binding protein)
• UniProt Protein Name: Growth hormone receptor
• Protein Family: Growth hormone receptor
• UniProt Gene Name: Ghr
• UniProt Synonym Gene Names: GH receptor; GH-binding protein; GHBP
• UniProt Entry Name: GHR_MOUSE

Uniprot Description

GH receptor: Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. Defects in GHR are a cause of Laron syndrome (LARS). A severe form of growth hormone insensitivity characterized by growth impairment, short stature, dysfunctional growth hormone receptor, and failure to generate insulin-like growth factor I in response to growth hormone. Defects in GHR may be a cause of idiopathic short stature autosomal (ISSA). Short stature is defined by a subnormal rate of growth. Belongs to the type I cytokine receptor family. Type 1 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Cellular Component: extracellular space; cell surface; mitochondrion; integral to plasma membrane; integral to membrane; extracellular region; extrinsic to membrane; cell soma; membrane; cytoplasm; plasma membrane; nucleus; receptor complex

Molecular Function: growth hormone receptor activity; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; protein homodimerization activity; growth factor binding; peptide hormone binding; SH2 domain binding; protein phosphatase binding; protein kinase binding

Biological Process: activation of JAK protein; succinate metabolic process; isoleucine metabolic process; oxaloacetate metabolic process; hormone-mediated signaling; activation of MAPK activity; cytokine and chemokine mediated signaling pathway; positive regulation of JAK-STAT cascade; regulation of multicellular organism growth; valine metabolic process; citrate metabolic process; endocytosis; positive regulation of multicellular organism growth; fatty acid metabolic process; JAK-STAT cascade; tyrosine phosphorylation of JAK2 protein; creatine metabolic process; response to estradiol stimulus; multicellular organismal metabolic process; cellular response to hormone stimulus; 2-oxoglutarate metabolic process; positive regulation of peptidyl-tyrosine phosphorylation; insulin-like growth factor receptor signaling pathway; allantoin metabolic process; creatinine metabolic process; taurine metabolic process