SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
TNFbeta/LTA recombinant protein
TNFbeta/LTA protein (His tag)
Applications
SDS-Page
Purity
> 90% pure
Synonyms
TNFbeta/LTA; TNFbeta/LTA protein (His tag); TNF B protein; TNFB protein; member 1) protein; Lymphotoxin alpha (TNF superfamily protein; CLTalpha protein; TNF superfamily member 1 protein; Lymphotoxin alpha; TNFSF 1 protein; DIF protein; Hemorrhagic factor protein; Coley's toxin protein; LT protein; Natural killer cytotoxic factor protein; Tumor necrosis factor beta protein; OTTHUMP00000037612 protein; LTA protein; NKCF protein; Lymphotoxin alpha protein; LT alpha protein; LTalpha protein; Tumor necrosis factor ligand superfamily member 1 protein; Necrosin protein; Tumor necrosis factor superfamily member 1 protein; Differentiation inducing factor protein; OTTHUMP00000037613 protein; TNFbeta protein; TNFSF1 protein; TNFbeta/LTA recombinant protein
Host
Human
Purity/Purification
> 90% pure
Form/Format
Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 30% glycerol, 0.1M NaCl, and 1mM DTT.
Concentration
0.5 mg/ml (varies by lot)
Sequence
35-205 amino acids: MGSSHHHHHH SSGLVPRGSH MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL
Applicable Applications for TNFbeta/LTA recombinant protein
SDS-PAGE
Application Notes
L929 cytotoxicity assay using actinomycin D Cell Number: 2 x 10,000 cells/well Incubation: 24hr after sample treatment Assay Media: 5% FBS media Cytokine Concentration: 12 pg/ml - 25 ng/ml Detection method: MTT assay
Protein Type
Recombinant
Biological Significance
TNF beta, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Recombinant TNF beta protein was expressed in E Coli and purified by using conventional chromatography techniques.
Expression System
E Coli
Tag/Conjugate
His tag
Preparation and Storage
Store at 4 degree C for short term storage (1/2 weeks). Aliquot and store at -20 degree C or - 70 degree C for long term storage. Avoid repeated freeze/thaw cycles.
SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
Related Product Information for TNFbeta/LTA recombinant protein
Purified recombinant Human TNFbeta/LTA protein
Product Categories/Family for TNFbeta/LTA recombinant protein
Similar Products
Product Notes
The TNFbeta/LTA (Catalog #AAA836549) is a Recombinant Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TNFbeta/LTA can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. L929 cytotoxicity assay using actinomycin D Cell Number: 2 x 10,000 cells/well Incubation: 24hr after sample treatment Assay Media: 5% FBS media Cytokine Concentration: 12 pg/ml - 25 ng/ml Detection method: MTT assay. Researchers should empirically determine the suitability of the TNFbeta/LTA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: 35-205 amino acids: MGSSHHHHHH SSGLVPRGSH MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL. It is sometimes possible for the material contained within the vial of "TNFbeta/LTA, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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