SDS-PAGE
(Coomassie Blue stained SDS-PAGE of EGF proteinFigure annotation denotes ug of protein loaded and % gel used.)
EGF active protein
EGF protein
Applications
SDS-Page
Purity
> 95% pure
Synonyms
EGF; EGF protein; Epidermal growth factor protein; Urogastrone protein; epidermal growth factor (beta-urogastrone); Pro-epidermal growth factor protein; EGF active protein
Host
Human
Purity/Purification
> 95% pure
Form/Format
Supplied as a liquid in PBS, pH 7.4.
Concentration
1 mg/ml (varies by lot)
Sequence
MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR
Sequence Length
89
Applicable Applications for EGF active protein
SDS-PAGE
Application Notes
Cell line: Balb-3T3 (Mus musculus (mouse)) Maintenance Condition: RPMI 1640 containing 10% FBS Assay medium: serum free RPMI 1640 Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) Serum Free Starvation: 24hr with RPMI1640 Incubation time: 24 hr (after sample treatment) Concentration range: 1 pg/ml - 1 ug/ml Detection method: BrdU assay
Protein Type
Recombinant
Biological Significance
Recombinant human epidermal growth factor(EGF) is a 6.3 kDa globular protein containing 54 amino acids residues, including 3 intra-molecular disulfide bonds. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. Recombinant EGF was expressed in E Coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Bioactivity
The ED50 for this effect is < 0.89 ng/ml. Measured in a cell proliferation assay using mouse Balb3T3 cell.
Expression System
E Coli
Preparation and Storage
Store at 4 degree C for short term storage (1/2 weeks). Aliquot and store at -20 degree C or - 70 degree C for long term storage. Avoid repeated freeze/thaw cycles.
SDS-PAGE
(Coomassie Blue stained SDS-PAGE of EGF proteinFigure annotation denotes ug of protein loaded and % gel used.)
SDS-PAGE
(Coomassie Blue stained SDS-PAGE of EGF proteinFigure annotation denotes ug of protein loaded and % gel used.)
Related Product Information for EGF active protein
Purified recombinant Human EGF protein
Product Categories/Family for EGF active protein
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
6.3 kDa (54aa), confirmed by MALDI-TOF.
NCBI Official Full Name
epidermal growth factor, partial
UniProt Protein Name
Epidermal growth factor
Protein Family
UniProt Gene Name
epidermal growth factor/ EGF
UniProt Entry Name
Q28867_HORSE
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Product Notes
The EGF epidermal growth factor/ egf (Catalog #AAA836477) is an Active Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's EGF can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Cell line: Balb-3T3 (Mus musculus (mouse)) Maintenance Condition: RPMI 1640 containing 10% FBS Assay medium: serum free RPMI 1640 Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) Serum Free Starvation: 24hr with RPMI1640 Incubation time: 24 hr (after sample treatment) Concentration range: 1 pg/ml - 1 ug/ml Detection method: BrdU assay. Researchers should empirically determine the suitability of the EGF epidermal growth factor/ egf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR. It is sometimes possible for the material contained within the vial of "EGF, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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