SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
GMCSF active protein
GMCSF protein
Gene Names
CSF2; GMCSF; GM-CSF
Applications
SDS-Page
Purity
> 95% pure
Synonyms
GMCSF; GMCSF protein; MGC131935 protein; Eosinophil Colony Stimulating Factor protein; CMCSF protein; Sargramostim protein; GM-CSF protein; Pluripoietin Alpha protein; Granulocyte Macrophage Colony Stimulating Factor protein; Colony-stimulating factor protein; Colony Stimulating Factor 2 protein; CSF protein; Colony stimulating factor protein; CSF 2 protein; CSF2 protein; CSF Alpha protein; Molgramostin protein; Granulocyte-macrophage colony-stimulating factor protein; MGC138897 protein; Granulocyte-macrophage colony stimulating factor Burst Promoting Activity protein; GMCSF active protein
Host
Human
Purity/Purification
> 95% pure
Form/Format
Supplied as a liquid in PBS, pH 7.4.
Concentration
1 mg/ml (varies by lot)
Sequence
MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE
Sequence Length
144
Applicable Applications for GMCSF active protein
SDS-PAGE
Application Notes
Cell line: TF1 (human erythroleukemic cell). Maintenance Condition: 10% FBS RPMI 1640 with GM-CSF Assay medium: 10% FBS RPMI 1640 without Gm-CSF Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) Starvation: 24hr, Assay medium Incubation time: 24 hr (after sample treatment) Concentration range: 0.048 ng/ml - 25 ng/ml Detection method: MTT assay
Protein Type
Recombinant
Biological Significance
Human GM-CSF is a 14.6 kDa globular protein consisting of 128 amino acid containing two disulfide bonds. GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. Recombinant human GM-CSF was expressed in E Coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Bioactivity
The ED50 for this effect is < 0.18 ng/ml. Measured in a cell proliferation assay using TF1 human erythroleukemic cells.
Endotoxin Levels
< 1.0 EU per ug of protein (determined by LAL method)
Expression System
E Coli
Preparation and Storage
Store at 4 degree C for short term storage (1/2 weeks). Aliquot and store at -20 degree C or - 70 degree C for long term storage. Avoid repeated freeze/thaw cycles.
SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
Related Product Information for GMCSF active protein
Purified recombinant Human GMCSF protein
Product Categories/Family for GMCSF active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
14.6 kDa (128 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
granulocyte-macrophage colony-stimulating factor, partial
NCBI Official Synonym Full Names
colony stimulating factor 2 (granulocyte-macrophage)
NCBI Official Symbol
CSF2
NCBI Official Synonym Symbols
GMCSF; GM-CSF
NCBI Protein Information
colony stimulating factor 2 (granulocyte-macrophage)
UniProt Protein Name
Granulocyte-macrophage colony-stimulating factor
UniProt Gene Name
GMCSF
UniProt Entry Name
Q5W4T7_CHICK
Research Articles on GMCSF
Similar Products
Product Notes
The GMCSF gmcsf (Catalog #AAA836763) is an Active Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GMCSF can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Cell line: TF1 (human erythroleukemic cell). Maintenance Condition: 10% FBS RPMI 1640 with GM-CSF Assay medium: 10% FBS RPMI 1640 without Gm-CSF Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) Starvation: 24hr, Assay medium Incubation time: 24 hr (after sample treatment) Concentration range: 0.048 ng/ml - 25 ng/ml Detection method: MTT assay. Researchers should empirically determine the suitability of the GMCSF gmcsf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE. It is sometimes possible for the material contained within the vial of "GMCSF, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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