IL2 active protein

IL2 protein

Cat. Num. AAA836386

• Gene Names: IL2; IL-2; TCGF; lymphokine
• Applications: SDS-Page
• Purity: > 95% pure
• Synonyms: IL2; IL2 protein; T-cell growth factor protein; Interleukin 2 protein; IL-2; IL-2 protein; IL 2 protein; Aldesleukin protein; Interleukin-2 protein; IL 2; TCGF protein; IL2 active protein

• Host: Human
• Purity/Purification: > 95% pure
• Form/Format: Supplied as a liquid in PBS, pH 7.4.
• Concentration: 1 mg/ml (varies by lot)
• Sequence: MPTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFSQSIIS TLT
• Sequence Length: 153

• Applicable Applications for IL2 active protein: SDS-PAGE
• Application Notes: Cell line: CTLL2 (mouse cytotoxic T-cell) Maintenance Condition: 10% FBS RPMI 1640 with hIL2 Assay medium: 10% FBS RPMI 1640 without hIL2 Cell Density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) Starvation: 24hr, Assay medium Incubation Time: 24hr (after sample treatment) Concentration Range: 0.039 ng/ml - 40 ng/ml Detection method: MTT assay
• Protein Type: Recombinant
• Biological Significance: IL-2, also known as T cell growth factor(TCGF), is an immunoregulatory lymphokine produced by T-cells in response to protein or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. This recombinant human IL-2 is a 15.4 kDa protein, containing 133 amino acid residues including one intra chain disulfide bond. Recombinant human IL-2 was overexpressed in E Coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
• Bioactivity: Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is < 1.5 ng/ml.
• Expression System: E Coli
• Preparation and Storage: Store at 4 degree C for short term storage (1/2 weeks). Aliquot and store at -20 degree C or - 70 degree C for long term storage. Avoid repeated freeze/thaw cycles.

SDS-PAGE

(Figure annotation denotes ug of protein loaded and % gel used.)

Related Product Information for IL2 active protein

Purified recombinant Human IL2 protein

Product Categories/Family for IL2 active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 28178861
• NCBI GeneID: 3558
• NCBI Accession #: NP_000577.2
• NCBI GenBank Nucleotide #: NM_000586.3
• UniProt Accession #: P60568; P01585
• Molecular Weight: 15.4 kDa (133aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: interleukin-2
• NCBI Official Synonym Full Names: interleukin 2
• NCBI Official Symbol: IL2
• NCBI Official Synonym Symbols: IL-2; TCGF; lymphokine
• NCBI Protein Information: interleukin-2
• UniProt Protein Name: Interleukin-2
• Protein Family: Interleukin
• UniProt Gene Name: IL2
• UniProt Synonym Gene Names: IL-2; TCGF
• UniProt Entry Name: IL2_HUMAN

NCBI Description

The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]

Uniprot Description

IL2: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells. A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. Belongs to the IL-2 family.

Protein type: Secreted, signal peptide; Cytokine; Secreted; Oncoprotein

Chromosomal Location of Human Ortholog: 4q26-q27

Cellular Component: extracellular space; extracellular region

Molecular Function: growth factor activity; interleukin-2 receptor binding; cytokine activity; kinase activator activity; carbohydrate binding; glycosphingolipid binding; kappa-type opioid receptor binding

Biological Process: positive regulation of isotype switching to IgG isotypes; negative regulation of heart contraction; natural killer cell activation; positive regulation of activated T cell proliferation; negative regulation of lymphocyte proliferation; elevation of cytosolic calcium ion concentration; negative regulation of protein amino acid phosphorylation; cell-cell signaling; positive regulation of cell proliferation; protein kinase C activation; positive regulation of B cell proliferation; cell adhesion; T cell differentiation; positive regulation of interleukin-17 production; regulation of T cell homeostatic proliferation; positive regulation of regulatory T cell differentiation; positive regulation of immunoglobulin secretion; positive regulation of cell growth; positive regulation of interferon-gamma production; positive regulation of tissue remodeling; positive regulation of tyrosine phosphorylation of Stat5 protein; negative regulation of inflammatory response; immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of B cell apoptosis; negative regulation of apoptosis; positive regulation of inflammatory response