Western Blot (WB)
(Anti-RBPJ Antibody (N-term)at 1:2000 dilution + human pancreas lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human RBPJ Polyclonal Antibody | anti-RBPJ antibody
RBPJ Antibody (N-term)
Western Blot (WB)
(Anti-RBPJ Antibody (N-term)at 1:2000 dilution + human pancreas lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-RBPJ Antibody (N-term)at 1:2000 dilution + human pancreas lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(RBPJ Antibody (N-term) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the RBPJ antibody detected the RBPJ protein (arrow).)
Western Blot (WB)
(RBPJ Antibody (N-term) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the RBPJ antibody detected the RBPJ protein (arrow).)
Immunohistochemistry (IHC)
(RBPJ Antibody (N-term) (MBS9204999)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RBPJ Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(RBPJ Antibody (N-term) (MBS9204999)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RBPJ Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Fluorescent confocal image of A2058 cell stained with RBPJ Antibody (N-term). A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RBPJ primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RBPJ immunoreactivity is localized to nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of A2058 cell stained with RBPJ Antibody (N-term). A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RBPJ primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RBPJ immunoreactivity is localized to nucleus significantly.)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Roberts, K.E., et al. Gastroenterology 139(1):130-139(2010)
Stahl, E.A., et al. Nat. Genet. 42(6):508-514(2010)
Johnson, S.E., et al. J. Biol. Chem. 285(9):6681-6692(2010)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a transcriptional regulator important in the Notch signaling pathway. The encoded protein acts as a repressor when not bound to Notch proteins and an activator when bound to Notch proteins. It is thought to function by recruiting chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to Notch signaling pathway genes. Several transcript variants encoding different isoforms have been found for this gene, and several pseudogenes of this gene exist on chromosome 9. [provided by RefSeq, Oct 2013]
Uniprot Description
RBPJ: Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA. Interacts with activated NOTCH1, NOTCH2 or NOTCH3. Interacts with MINT/SHARP. This interaction may mediate the recruitment of large corepressor complexes containing proteins such as HDAC1, HDAC2, NCOR2, SAP30, FHL1/KYOT2 and CIR1. Interacts with EP300, MAML1 and PTF1A. Interacts with Epstein-Barr virus EBNA2, EBNA3, EBNA4 and EBNA6. Interacts with RITA/C12orf52, leading to nuclear export, prevent the interaction between RBPJ and NICD product and subsequent down-regulation of the Notch signaling pathway. Belongs to the Su(H) family. 7 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell development/differentiation; Transcription factor
Chromosomal Location of Human Ortholog: 4p15.2
Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleolus; nucleus
Molecular Function: protein binding; DNA binding; recombinase activity; protein N-terminus binding; chromatin binding; transcription factor activity
Biological Process: transcription initiation from RNA polymerase II promoter; Notch signaling pathway; epidermal cell fate specification; somatic stem cell maintenance; sebaceous gland development; myeloid dendritic cell differentiation; negative regulation of transcription from RNA polymerase II promoter; hair follicle maturation; humoral immune response; activation of Notch receptor target transcription factor; DNA recombination; keratinocyte differentiation; negative regulation of cell proliferation; inflammatory response to antigenic stimulus; pituitary gland development; B cell differentiation; defense response to bacterium; negative regulation of ossification; epithelial to mesenchymal transition; gene expression; positive regulation of transcription from RNA polymerase II promoter; blood vessel remodeling; angiogenesis; negative regulation of transcription, DNA-dependent; auditory receptor cell fate commitment; regulation of timing of cell differentiation; positive regulation of BMP signaling pathway; positive regulation of cardiac muscle cell proliferation
Disease: Adams-oliver Syndrome 3
Research Articles on RBPJ
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Product Notes
The RBPJ rbpj (Catalog #AAA9204999) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-29. The RBPJ Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RBPJ can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF). WB~~1:1000. Researchers should empirically determine the suitability of the RBPJ rbpj for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RBPJ, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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