SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
TNFalpha recombinant protein
TNFalpha protein
Applications
SDS-Page
Purity
> 95% pure
Synonyms
TNFalpha; TNFalpha protein; TNF protein; TNFA protein; TNF-alpha protein; TNFSF2 protein; Tumor necrosis factor alpha protein; CACHECTIN protein; DIF (differentiation inducing factor) protein; DIF protein; member 2) protein; TNF superfamily member 2 protein; tumor necrosis factor (TNF superfamily protein; TNFalpha recombinant protein
Host
Human
Purity/Purification
> 95% pure
Form/Format
Supplied as a liquid in PBS, pH 7.4.
Concentration
1 mg/ml (varies by lot)
Sequence
MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
Applicable Applications for TNFalpha recombinant protein
SDS-PAGE
Application Notes
L929 cytotoxicity assay using actinomycin D Cell Number: 2 x 10,000 cells/well
Incubation: 24hr after sample treatment
Assay Media: 5% FBS media
Cytokine Concentration: 12 pg/ml - 25 ng/ml
Detection method: MTT assay
Incubation: 24hr after sample treatment
Assay Media: 5% FBS media
Cytokine Concentration: 12 pg/ml - 25 ng/ml
Detection method: MTT assay
Protein Type
Recombinant
Biological Significance
Tumor necrosis factor alpha (TNF-alpha), also called cachectin, consists of 157 amino acids. TNF-alpha is a 17.5 kDa factor produced by neutrophils, CD4+ T cells, macrophage, NK cells, LAK cells, astrocytes endothelial cells, and smooth muscle cells. TNF-alpha is cytolytic and plays an important role in immune regulation including hemorrhagic tumor necrosis/cytotoxicity and inflammation, and in regulation of antiviral and immune proliferative and activation responses. The active form of this protein is a trimer. Recombinant human TNF-alpha was expressed in E Coli and purified by using conventional chromatography techniques. Additional amino acid, methionine, was attached at N-terminus of the protein.
Endotoxin Levels
< 1.0 EU per ug of protein (determined by LAL method)
Expression System
E Coli
Preparation and Storage
Store at 4 degree C for short term storage (1/2 weeks). Aliquot and store at -20 degree C or - 70 degree C for long term storage. Avoid repeated freeze/thaw cycles.
SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
Related Product Information for TNFalpha recombinant protein
Purified recombinant Human TNFalpha protein
Product Categories/Family for TNFalpha recombinant protein
Similar Products
Product Notes
The TNFalpha (Catalog #AAA536759) is a Recombinant Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TNFalpha can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. L929 cytotoxicity assay using actinomycin D Cell Number: 2 x 10,000 cells/well Incubation: 24hr after sample treatment Assay Media: 5% FBS media Cytokine Concentration: 12 pg/ml - 25 ng/ml Detection method: MTT assay. Researchers should empirically determine the suitability of the TNFalpha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL. It is sometimes possible for the material contained within the vial of "TNFalpha, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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