Tissue Inhibitors Of Metalloproteinase 4 (TIMP4) Recombinant Protein | TIMP4 recombinant protein

Recombinant Tissue Inhibitors Of Metalloproteinase 4 (TIMP4)

Cat. Num. AAA2009202

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Tissue Inhibitors Of Metalloproteinase 4 (TIMP4); Recombinant Tissue Inhibitors Of Metalloproteinase 4 (TIMP4); TIMP4 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF- H VCHSALVIRA KISSEKVVPA SEDPADTQKM IRYEIKQIKM FKGFEKAKDI QYVYTPFDSS LCGVKLETNS QKQYLLTGQI LSDGKVFIHL CNYIEPWEDL SLVQRESLNH HYHQNCGCQI TTCYAVPCTI SAPDECLWTD WLLERKLYGY QAQHYVCMKH VDGICSWYRG HLHLRKEYVD IVQP
• Sequence Length: 224

• Applicable Applications for TIMP4 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: His40~Pro224 (Accession # P81556) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 157820749
• NCBI GeneID: 680130
• NCBI Accession #: NP_001102863.1
• NCBI GenBank Nucleotide #: NM_001109393.1
• UniProt Accession #: P81556
• Molecular Weight: 23.1kDa
• NCBI Official Full Name: metalloproteinase inhibitor 4
• NCBI Official Synonym Full Names: tissue inhibitor of metalloproteinase 4
• NCBI Official Symbol: Timp4
• NCBI Protein Information: metalloproteinase inhibitor 4; TIMP-4; tissue inhibitor of metalloproteinases 4
• UniProt Protein Name: Metalloproteinase inhibitor 4
• Protein Family: Metalloproteinase inhibitor
• UniProt Gene Name: Timp4
• UniProt Synonym Gene Names: TIMP-4
• UniProt Entry Name: TIMP4_RAT

NCBI Description

regulator of the matrix metalloproteinases; involved in regulating MMPs in follicles and corpus luteum [RGD, Feb 2006]

Uniprot Description

Function: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.

Subcellular location: Secreted.

Tissue specificity: Expressed in retina, smooth muscle, skin, pancreas, skeletal muscle, heart, brain, lung, kidney and testis. Not found in cartilage, spleen and liver.

Sequence similarities: Belongs to the protease inhibitor I35 (TIMP) family. [View classification]Contains 1 NTR domain.