Tissue Inhibitors Of Metalloproteinase 4 Recombinant Protein | TIMP4 recombinant protein

Recombinant Tissue Inhibitors Of Metalloproteinase 4 (TIMP4)

Cat. Num. AAA2029092

• Gene Names: Timp4; TIMP-4
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Tissue Inhibitors Of Metalloproteinase 4; Recombinant Tissue Inhibitors Of Metalloproteinase 4 (TIMP4); TIMP4 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 8.1 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- A KISSEKVVPA STDPADPQKM IRYEIKQIKM FKGFEKVNDI QYIYTPFDSS LCGVKLEANS QKRYLLTGQI LSDGKVFVHL CNYIEPWENL SFLQRESLNH HYHLNCGCQI TTCYAVPCTI SAPNECLWTD WLLERKLYGYQAQHYVCMKH VDGSCSWYQG RLPLRKEFVD IIQP
• Sequence Length: 51

• Applicable Applications for TIMP4 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 24.2kDa
• Accurate Molecular Mass (KD): 27kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Bos taurus; Bovine (Cattle)
• Fragment: Ala50~Pro224
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 48734400
• NCBI GeneID: 110595
• Molecular Weight: 25,774 Da
• NCBI Official Full Name: tissue inhibitor of metalloproteinase 4, partial
• NCBI Official Synonym Full Names: tissue inhibitor of metalloproteinase 4
• NCBI Official Symbol: Timp4
• NCBI Official Synonym Symbols: TIMP-4
• NCBI Protein Information: metalloproteinase inhibitor 4
• UniProt Protein Name: Metalloproteinase inhibitor 4
• Protein Family: Metalloproteinase inhibitor
• UniProt Gene Name: Timp4
• UniProt Synonym Gene Names: TIMP-4
• UniProt Entry Name: TIMP4_MOUSE

Uniprot Description

TIMP4: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP- 9. Belongs to the protease inhibitor I35 (TIMP) family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: proteinaceous extracellular matrix; extracellular space; sarcomere; extracellular region

Molecular Function: enzyme inhibitor activity; metalloendopeptidase inhibitor activity; protease binding; protease inhibitor activity; metal ion binding

Biological Process: negative regulation of metalloenzyme activity; response to cytokine stimulus; response to hormone stimulus; negative regulation of peptidase activity; negative regulation of membrane protein ectodomain proteolysis