Rabbit SATB2 Polyclonal Antibody | anti-SATB2 antibody

Rabbit Polyclonal to Human SATB2

Cat. Num. AAA243944

• Gene Names: SATB2; GLSS
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: SATB2; Polyclonal Antibody; Rabbit Polyclonal to Human SATB2; Anti-SATB2 Antibody (C-Terminus) IHC-plus; DNA-binding protein SATB2; KIAA1034; SATB family member 2; SATB homeobox 2; Human SATB2; anti-SATB2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 733

• Applicable Applications for anti-SATB2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (5 ug/ml), WB (2 - 4 ug/ml)
• Target Species: Human
• Immunogen Description: 14 amino acid peptide near the carboxy terminus of the human SATB2.
• Immunogen: SATB2 antibody was raised against 14 amino acid peptide near the carboxy terminus of the human SATB2.
• Antigen Modification: C-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-SATB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of SATB2 in Human Brain cells with SATB2 antibody at 50 ug/ml.)

Western Blot (WB)

(Western blot of SATB2 in A20 cell lysate with SATB2 antibody at (A) 2 and (B) 4 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 289547596
• NCBI GeneID: 23314
• NCBI Accession #: NP_001165980.1
• NCBI GenBank Nucleotide #: NM_001172509.1
• UniProt Accession #: Q9UPW6; Q3ZB87; Q4V763; A8K5Z8
• Molecular Weight: 69,137 Da
• NCBI Official Full Name: DNA-binding protein SATB2
• NCBI Official Synonym Full Names: SATB homeobox 2
• NCBI Official Symbol: SATB2
• NCBI Official Synonym Symbols: GLSS
• NCBI Protein Information: DNA-binding protein SATB2; SATB family member 2; special AT-rich sequence-binding protein 2
• UniProt Protein Name: DNA-binding protein SATB2
• Protein Family: DNA-binding protein
• UniProt Gene Name: SATB2
• UniProt Synonym Gene Names: KIAA1034
• UniProt Entry Name: SATB2_HUMAN

NCBI Description

This gene encodes a DNA binding protein that specifically binds nuclear matrix attachment regions. The encoded protein is involved in transcription regulation and chromatin remodeling. Defects in this gene are associated with isolated cleft palate and mental retardation. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Feb 2010]

Uniprot Description

SATB2: Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper- layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation. Chromosomal aberrations involving SATB2 are found in isolated cleft palate. Translocation t(2;7); translocation t(2;11). Defects in SATB2 are a cause of cleft palate isolated (CPI). A congenital fissure of the soft and/or hard palate, due to faulty fusion. Isolated cleft palate is not associated with cleft lips. Some patients may manifest other craniofacial dysmorphic features, mental retardation, and osteoporosis. A chromosomal aberration involving SATB2 is found in a patient with classical features of Toriello-Carey syndrome. Translocation t(2;14)(q33;q22). Belongs to the CUT homeobox family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: nucleoplasm; transcription factor complex; nuclear matrix; cytoplasm; histone deacetylase complex

Molecular Function: protein binding; sequence-specific DNA binding; chromatin binding

Biological Process: chromatin remodeling; transcription, DNA-dependent; commitment of a neuronal cell to a specific type of neuron in the forebrain; cartilage development; neuron migration; positive regulation of transcription from RNA polymerase II promoter; palate development; negative regulation of transcription from RNA polymerase II promoter; embryonic pattern specification; osteoblast development; embryonic skeletal morphogenesis

Disease: Glass Syndrome