Rabbit anti-Human DDX56 Polyclonal Antibody | anti-DDX56 antibody

DDX56 Rabbit pAb

Cat. Num. AAA4758709

• Gene Names: DDX56; DDX21; DDX26; NOH61
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: DDX56; Polyclonal Antibody; DDX56 Rabbit pAb; DDX21; DDX26; NOH61; anti-DDX56 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: -20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

• Applicable Applications for anti-DDX56 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:200-1:1000
• Immunogen: Synthetic peptide of human DDX56
• Conjugation: Unconjugated
• Molecular Function: This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene shows ATPase activity in the presence of polynucleotides and associates with nucleoplasmic 65S preribosomal particles. This gene may be involved in ribosome synthesis, most likely during assembly of the large 60S ribosomal subunit. Multiple transcript variants encoding different isoforms have been found for this gene.
• Modification: Unmodified
• Cell Position: Nucleus
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-3: Hela, 293T and HEPG2 cell lysates, Primary antibody: 863683(DDX56 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 7 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 863683(DDX56 Antibody) at dilution 1/20. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 863683(DDX56 Antibody) at dilution 1/20. (Original magnification: ×200))

Product Categories/Family for anti-DDX56 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 9506931
• NCBI GeneID: 54606
• NCBI Accession #: NP_061955
• NCBI GenBank Nucleotide #: NM_019082.3
• UniProt Accession #: Q9NY93; Q6IAE2; Q9H9I8; A4D2K9; C9JV95
• Molecular Weight: 64.0 kDa (570aa)
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX56 isoform 1
• NCBI Official Synonym Full Names: DEAD-box helicase 56
• NCBI Official Symbol: DDX56
• NCBI Official Synonym Symbols: DDX21; DDX26; NOH61
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX56
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX56
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX56
• UniProt Synonym Gene Names: DDX21; NOH61
• UniProt Entry Name: DDX56_HUMAN

NCBI Description

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene shows ATPase activity in the presence of polynucleotides and associates with nucleoplasmic 65S preribosomal particles. This gene may be involved in ribosome synthesis, most likely during assembly of the large 60S ribosomal subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]

Uniprot Description

DDX56: May play a role in later stages of the processing of the pre-ribosomal particles leading to mature 60S ribosomal subunits. Has intrinsic ATPase activity. Belongs to the DEAD box helicase family. DDX56/DBP9 subfamily.

Protein type: Nucleolus; Helicase; EC 3.6.4.13

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: membrane; nucleolus

Molecular Function: ATP binding; ATP-dependent RNA helicase activity

Biological Process: rRNA processing