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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Rat Tissue Inhibitors Of Metalloproteinase 4 (TIMP4) Polyclonal Antibody | anti-TIMP4 antibody

Polyclonal Antibody to Tissue Inhibitors Of Metalloproteinase 4 (TIMP4)

Gene Names
Timp4; Timp-4
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Tissue Inhibitors Of Metalloproteinase 4 (TIMP4); Polyclonal Antibody; Polyclonal Antibody to Tissue Inhibitors Of Metalloproteinase 4 (TIMP4); anti-TIMP4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against TIMP4. It has been selected for its ability to recognize TIMP4 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF- H VCHSALVIRA KISSEKVVPA SEDPADTQKM IRYEIKQIKM FKGFEKAKDI QYVYTPFDSS LCGVKLETNS QKQYLLTGQI LSDGKVFIHL CNYIEPWEDL SLVQRESLNH HYHQNCGCQI TTCYAVPCTI SAPDECLWTD WLLERKLYGY QAQHYVCMKH VDGICSWYRG HLHLRKEYVD IVQP
Sequence Length
51
Applicable Applications for anti-TIMP4 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant TIMP4 (His40~Pro224) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2038407
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Glioma Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Rat Glioma Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,723 Da
NCBI Official Full Name
metalloproteinase inhibitor 4
NCBI Official Synonym Full Names
TIMP metallopeptidase inhibitor 4
NCBI Official Symbol
Timp4
NCBI Official Synonym Symbols
Timp-4
NCBI Protein Information
metalloproteinase inhibitor 4
UniProt Protein Name
Metalloproteinase inhibitor 4
UniProt Gene Name
Timp4
UniProt Synonym Gene Names
TIMP-4

NCBI Description

regulator of the matrix metalloproteinases; involved in regulating MMPs in follicles and corpus luteum [RGD, Feb 2006]

Uniprot Description

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.

Research Articles on TIMP4

Similar Products

Product Notes

The TIMP4 timp4 (Catalog #AAA2006721) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Tissue Inhibitors Of Metalloproteinase 4 (TIMP4) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Tissue Inhibitors Of Metalloproteinase 4 (TIMP4) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the TIMP4 timp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- H VCHSALVIRA KISSEKVVPA SEDPADTQKM IRYEIKQIKM FKGFEKAKDI QYVYTPFDSS LCGVKLETNS QKQYLLTGQI LSDGKVFIHL CNYIEPWEDL SLVQRESLNH HYHQNCGCQI TTCYAVPCTI SAPDECLWTD WLLERKLYGY QAQHYVCMKH VDGICSWYRG HLHLRKEYVD IVQP. It is sometimes possible for the material contained within the vial of "Tissue Inhibitors Of Metalloproteinase 4 (TIMP4), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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