Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) Recombinant Protein | TIMP3 recombinant protein

Recombinant Tissue Inhibitors Of Metalloproteinase 3 (TIMP3)

Cat. Num. AAA2009685

• Gene Names: Timp3; Timp-3
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Tissue Inhibitors Of Metalloproteinase 3 (TIMP3); Recombinant Tissue Inhibitors Of Metalloproteinase 3 (TIMP3); TIMP3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-GAEACTCSPS HPQDAFCNSD IVIRAKVVGK KLVKEGPFGT LVYTIKQMKM YRGFSKMPHV QYIHTEASES LCGLKLEVNK YQYLLTGRVY EGKMYTGLCN FVERWDHLTL SQRKGLNYRY HLGCNCKIKS CYYLPCFVTS KNECLWTDML SNFGYPGYQS KHYACIRQKG GYCSWYRGWA PPDKSISNAT DP
• Sequence Length: 211

• Applicable Applications for TIMP3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Gly20~Pro211 (Accession # P39876) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 6755793
• NCBI GeneID: 21859
• NCBI Accession #: NP_035725.1
• NCBI GenBank Nucleotide #: NM_011595.2
• UniProt Accession #: P39876; Q91WL9
• Molecular Weight: 23.5kDa
• NCBI Official Full Name: metalloproteinase inhibitor 3
• NCBI Official Synonym Full Names: tissue inhibitor of metalloproteinase 3
• NCBI Official Symbol: Timp3
• NCBI Official Synonym Symbols: Timp-3
• NCBI Protein Information: metalloproteinase inhibitor 3; tissue inhibitor of metalloproteinases 3
• UniProt Protein Name: Metalloproteinase inhibitor 3
• Protein Family: Metalloproteinase inhibitor
• UniProt Gene Name: Timp3
• UniProt Synonym Gene Names: Sun; Timp-3; TIMP-3
• UniProt Entry Name: TIMP3_MOUSE

Uniprot Description

TIMP3: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15. Interacts with EFEMP1. Belongs to the protease inhibitor I35 (TIMP) family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; cytoplasm; extracellular region; basement membrane; nucleus

Molecular Function: enzyme inhibitor activity; metalloendopeptidase inhibitor activity; protease binding; protease inhibitor activity; metal ion binding

Biological Process: negative regulation of proteolysis; negative regulation of metalloenzyme activity; response to cytokine stimulus; response to hormone stimulus; negative regulation of peptidase activity; negative regulation of membrane protein ectodomain proteolysis