Chicken Nuclear RNA Export Factor 1 ELISA Kit | NXF1 elisa kit

Chicken Nuclear RNA Export Factor 1 ELISA Kit

Cat. Num. AAA744701

• Gene Names: NXF1; TAP; MEX67
• Reactivity: Chicken
• Synonyms: Nuclear RNA Export Factor 1; Chicken Nuclear RNA Export Factor 1 ELISA Kit; NXF1 elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NXF1 elisa kit

Intended Uses: This NXF1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken NXF1. This ELISA kit for research use only!

Principle of the Assay: NXF1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NXF1 antibody and an NXF1-HRP conjugate. The assay sample and buffer are incubated together with NXF1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NXF1 concentration since NXF1 from samples and NXF1-HRP conjugate compete for the anti-NXF1 antibody binding site. Since the number of sites is limited, as more sites are occupied by NXF1 from the sample, fewer sites are left to bind NXF1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NXF1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for NXF1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4220554
• NCBI GeneID: 10482
• UniProt Accession #: Q9UBU9; Q99799; Q9UQL2; B4E269
• Molecular Weight: 40,476 Da
• NCBI Official Full Name: nuclear RNA export factor 1
• NCBI Official Synonym Full Names: nuclear RNA export factor 1
• NCBI Official Symbol: NXF1
• NCBI Official Synonym Symbols: TAP; MEX67
• NCBI Protein Information: nuclear RNA export factor 1; mRNA export factor TAP; tip-associated protein; tip associating protein; tip-associating protein
• UniProt Protein Name: Nuclear RNA export factor 1
• Protein Family: Nuclear RNA export factor
• UniProt Gene Name: NXF1
• UniProt Synonym Gene Names: TAP
• UniProt Entry Name: NXF1_HUMAN

NCBI Description

This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

NXF1: Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5. Belongs to the NXF family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 11q12-q13

Cellular Component: nucleoplasm; nuclear speck; nuclear pore; cytosol; nuclear inclusion body

Molecular Function: mRNA binding; protein binding; nucleotide binding; nucleocytoplasmic transporter activity

Biological Process: poly(A)+ mRNA export from nucleus; mRNA export from nucleus; viral reproduction; gene expression