Human apolipoprotein O ELISA Kit | APOO elisa kit

Human Apolipoprotein O, APOO ELISA Kit

Cat. Num. AAA910094

• Gene Names: APOO; My025; FAM121B
• Reactivity: Human
• Synonyms: apolipoprotein O; Human Apolipoprotein O; APOO ELISA Kit; Human Apolipoprotein O (APOO) ELISA kit; FAM121B; MGC4825; My025; brain my025; family with sequence similarity 121B; APOO elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human APOO. No significant cross-reactivity or interference between Human APOO and analogues was observed.
• Sequence Length: 198

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Competitive
• Detection Range: 0.31 ng/ml-20 ng/ml
• Sensitivity: 0.08 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Transport
• Protein Biological Process 3: Lipid transport
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOO elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with APOO. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for APOO. The competitive inhibition reaction is launched between with pre-coated APOO and APOO in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of APOO in the samples. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 13129148
• NCBI GeneID: 79135
• NCBI Accession #: NP_077027.1
• NCBI GenBank Nucleotide #: NM_024122.4
• UniProt Accession #: Q9BUR5; Q9H3J9; B2R4K9
• Molecular Weight: 22,285 Da
• NCBI Official Full Name: apolipoprotein O
• NCBI Official Synonym Full Names: apolipoprotein O
• NCBI Official Symbol: APOO
• NCBI Official Synonym Symbols: My025; FAM121B
• NCBI Protein Information: apolipoprotein O; brain my025; family with sequence similarity 121B
• UniProt Protein Name: Apolipoprotein O
• Protein Family: MICOS complex
• UniProt Gene Name: APOO
• UniProt Synonym Gene Names: FAM121B; My025; UNQ1866/PRO4302
• UniProt Entry Name: APOO_HUMAN

NCBI Description

This gene is a member of the apolipoprotein family. Members of this protein family are involved in the transport and metabolism of lipids. The encoded protein associates with HDL, LDL and VLDL lipoproteins and is characterized by chondroitin-sulfate glycosylation. This protein may be involved in preventing lipid accumulation in the myocardium in obese and diabetic patients. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 3, 4, 5, 12 and 16.[provided by RefSeq, Sep 2009]

Uniprot Description

APOO: Promotes cholesterol efflux from macrophage cells. Detected in HDL, LDL and VLDL. Secreted by a microsomal triglyceride transfer protein (MTTP)-dependent mechanism, probably as a VLDL-associated protein that is subsequently transferred to HDL. May be involved in myocardium-protective mechanisms against lipid accumulation. Belongs to the apolipoprotein O family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.11

Cellular Component: extracellular space; mitochondrion; integral to membrane

Biological Process: lipid transport