Mouse Thyroglobulin ELISA Kit | TG elisa kit

Mouse Thyroglobulin ELISA Kit

Cat. Num. AAA726270

• Gene Names: TG; TGN; AITD3
• Reactivity: Mouse
• Synonyms: Thyroglobulin; Mouse Thyroglobulin ELISA Kit; TG elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This TG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse TG. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TG elisa kit

Principle of the Assay: TG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TG antibody and an TG-HRP conjugate. The assay sample and buffer are incubated together with TG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TG concentration since TG from samples and TG-HRP conjugate compete for the anti-TG antibody binding site. Since the number of sites is limited, as more sites are occupied by TG from the sample, fewer sites are left to bind TG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TG concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 37174
• NCBI GeneID: 7038
• UniProt Accession #: P01266; O15274; O43899; Q15593; Q15948; Q9NYR1; Q9NYR2; Q9UMZ0; Q9UNY3
• Molecular Weight: 298,383 Da
• NCBI Official Full Name: thyroglobulin
• NCBI Official Synonym Full Names: thyroglobulin
• NCBI Official Symbol: TG
• NCBI Official Synonym Symbols: TGN; AITD3
• NCBI Protein Information: thyroglobulin
• UniProt Protein Name: Thyroglobulin
• Protein Family: Thyroglobulin
• UniProt Gene Name: TG
• UniProt Synonym Gene Names: Tg
• UniProt Entry Name: THYG_HUMAN

NCBI Description

Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis. [provided by RefSeq, Nov 2009]

Uniprot Description

TG: Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). Defects in TG are the cause of thyroid dyshormonogenesis 3 (TDH3). A disorder due to thyroid dyshormonogenesis, causing large goiters of elastic and soft consistency in the majority of patients. Although the degree of thyroid dysfunction varies considerably among patients with defective thyroglobulin synthesis, patients usually have a relatively high serum free triiodothyronine (T3) concentration with disproportionately low free tetraiodothyronine (T4) level. The maintenance of relatively high free T3 levels prevents profound tissue hypothyroidism except in brain and pituitary, which are dependent on T4 supply, resulting in neurologic and intellectual defects in some cases. Variations in TG are associated with susceptibility to autoimmune thyroid disease type 3 (AITD3). AITDs including Graves disease (GD) and Hashimoto thyroiditis (HT), are among the most common human autoimmune diseases. They are complex diseases, which are caused by an interaction between susceptibility genes and nongenetic factors, such as infection. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8q24

Cellular Component: extracellular space; extracellular region

Molecular Function: hormone activity

Biological Process: regulation of myelination; iodide transport; thyroid gland development; hormone biosynthetic process; signal transduction; thyroid hormone metabolic process

Disease: Thyroid Dyshormonogenesis 3; Autoimmune Thyroid Disease, Susceptibility To, 3